Microelectrophoresis

Microelectrophoresis is a technique used to measure the movement of nanoparticles in a dilute dispersion when they are subjected to an electric field. It is based on the measurement of the electrophoretic mobility of the particles in the presence and absence of a polymer layer. The working principle of microelectrophoresis is based on laser Doppler microelectrophoresis, where molecules and particles move electrophoretically under the influence of an applied electric field, and the speed of their movement is directly related to the zeta potential.From: Nanoscience [2010], Surfactant Science and Technology [2019], Effect of modified γ-Fe₂O₃ magnetic nanoparticles on the stability of heavy oil-in-water emulsions [2023]

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Quantification and Elucidation of the Overall Interaction between Nanoparticles

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Published in Victor M. Starov, Nanoscience, 2010

W. Richard Bowen, Paul M. Williams

The zeta potential (and thus by calculation charge) can be obtained from electrophoretic mobility measurements [4]. Microelectrophoresis is the measurement of the movement of nanoparticles, in dilute dispersion, when they are placed in an electric field. The measurement can be used to determine the electrophoretic mobility, which is related to the zeta potential (and surface charge) [4].

Ultrasound-assisted synthesis of oil-in-water nanoemulsions: stability and rheological characteristics

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Published in Journal of Dispersion Science and Technology, 2023

Diksha Vats, Vimal Kumar

The measurements for droplet size, zeta potential, and electrophoretic mobility were conducted using a nanoparticle analyzer (Horiba, SZ-100). A Dynamic light scattering technique was used for droplet size measurements and a Laser Doppler microelectrophoresis (LDME) technique was used for zeta potential and electrophoretic measurements. Every measurement was carried out in triplicate. For preventing multiple scattering during measurements, samples were diluted to 100 times. To measure the nanoemulsion droplet diameter in the diluted samples, a technique called Photon correlation spectroscopy (PCS) was used. PCS technique works by utilizing the fluctuation intensity of particles created during the scattering of light. The suspended nanoemulsion droplets move due to Brownian motion.[23] The bigger the droplets of dispersed phases, lesser will be the Brownian motion of nanoemulsion droplets. The fluctuation intensity varies rapidly for smaller droplets of dispersed phase. It was measured by correlator; a correlation function was formed by correlator. After normalizing, the correlation function versus delay time plot could be obtained. The average droplet diameter of dispersed phase can be obtained from the cumulated analysis of the sample.[26]