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Electromagnetic Field Effects on Soft Tissues – Muscles and Tendons
Published in Marko S. Markov, James T. Ryaby, Erik I. Waldorff, Pulsed Electromagnetic Fields for Clinical Applications, 2020
Erik I. Waldorff, Nianli Zhang, James T. Ryaby, Andrew F. Kuntz
Using rodent neonatal ventricular myocytes in serum-free medium, Wei, Sun et al. (2015) assessed cell viability and intracellular free calcium concentration when exposed to caffeine (10 nM for 60 s) or EMF (0, 15, 50, 75, and 100 Hz at 2.0 mT for 180 s) using Fura-2-acetoxymethyl ester (Fura-2/AM) and spectrofluorometry. The former served as a control for the Na+/Ca2+ exchanger (NCX) function and activity of the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA). While caffeine application expectedly caused a rapid increase in intracellular Ca2+, EMF (all frequencies) was able to increase the intracellular Ca2+ transient baseline and reduce the amplitude of Ca2+ transients (i.e., a reduction in half-decay times for both caffeine-induced intracellular Ca2+ transients and spontaneous intracellular Ca2+ transients) and Ca2+ levels in the sarco/endoplasmic reticulum. Although the authors indicate that the mechanisms behind the effect of PEMF on NCX and SERCA activity are unknown, the data suggests that EMF can change intracellular Ca2+ signaling in cardiomyocytes.
Apigenin attenuates tetrabromobisphenol A-induced cytotoxicity in neuronal SK-N-MC cells
Published in Journal of Environmental Science and Health, Part A, 2023
Eun Mi Choi, So Young Park, Kwang Sik Suh, Suk Chon
Cells were seeded into black 96-well optical bottom cell culture plates (104 cells/well) 1 d before treatment. After rinsing with warm Dulbecco’s phosphate-buffered saline (DPBS), cells were pre-incubated for 1 h with a medium containing 1% (v/v) FBS and 0.01–1 μM apigenin before treatment with 20 μM TBBPA for 48 h. Cells were treated with 5 μM Fura-2-acetoxymethyl ester (Fura-2AM; Molecular Probes, Eugene, OR, USA) for 1 h at 37 °C. After washing with DPBS, fluorescence emission (510 nm) was monitored at excitation wavelengths of 340 and 380 nm using a fluorescence microplate reader (Molecular Devices, Sunnyvale, CA, USA). Relative changes in [Ca2+]i were reported as 340:380 nm fluorescence ratios.
Biochanin A prevents 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced adipocyte dysfunction in cultured 3T3-L1 cells
Published in Journal of Environmental Science and Health, Part A, 2019
Eun Mi Choi, Kwang Sik Suh, So Young Park, Sang Ouk Chin, Sang Youl Rhee, Suk Chon
Cells were treated with 5 μM Fura-2-acetoxymethyl ester (Fura-2AM; Molecular Probes, Eugene, OR, USA) for 1 h at 37 °C. Following a wash in PBS, fluorescence emissions (510 nm) were monitored at excitation wavelengths of 340 and 380 nm using a fluorescence microplate reader (Molecular Devices, Sunnyvale, CA, USA). Relative changes in [Ca2+]i concentrations are reported as the 340:380 nm fluorescence ratios.