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Titrimetric Analysis
Published in Pradyot Patnaik, Handbook of Environmental Analysis, 2017
Redox titrations find numerous applications in environmental analysis. Iodometric titration involving the reaction of iodine with a reducing agent such as thiosulfate or phenylarsine oxide (PAO) of known strength is a typical example of a redox titration. This method is discussed separately in the next section. Another example of redox titration is the determination of sulfite (SO32−) using ferric ammonium sulfate, [NH4Fe(SO4)2].
Common Acid, Alkali, and Other Standard Solutions
Published in James P. Lodge, Methods of Air Sampling and Analysis, 2017
Measure accurately about 40 mL of freshly standardized 0.1 N silver nitrate (AgNO3), and transfer to a 250-mL conical flask. Add 50 mL of water, swirl to mix the solution, and add 2 mL of nitric acid (HNO3) and 1 mL of ferric ammonium sulfate solution (FeNH4 (SO4)2·12H2O, 80 g/L). Titrate the AgNO3 solution with the NH4SCN solution until the first permanent reddish-brown color appears and persists after vigorous shaking for 1 min.
Frequency-dependent changes in mitochondrial number and generation of reactive oxygen species in a rat model of vibration-induced injury
Published in Journal of Toxicology and Environmental Health, Part A, 2020
To roughly estimate the mitochondrial number, Heidenhain’s hematoxylin stain was used. This particular hematoxylin stains chromatin, mitochondria, striations in skeletal muscle and myelin (Kudryavtseva et al. 2016; Wilson and Gamble 2002). The staining procedure was performed using one slide from the VTA, VTN, and skin. Each slide was fixed in 4% paraformaldehyde in 0.1M phosphate-buffered saline (PBS, pH 7.4) for 5 min, rinsed 4 × 5 min in distilled water (dH2O), and then stained. To perform the staining, sections were first incubated in a 5% ferric ammonium sulfate solution (in dH2O) for 1 hr., rinsed 4 × 5 min in dH2O, incubated in Heidenhain’s hematoxylin (0.5% hematoxylin, 10 ml 100% ethanol in 100 ml dH2O) for 1 hr, rinsed, and re-incubated in the ferric ammonium sulfate solution 30 min. Slides were then rinsed 2 × 1 min in dH20, dehydrated in an ascending series of 70%, 90%, and 100% ethanol, and after air-drying were cover slipped using Permount Mounting medium (VWR).