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Membrane-Based Separation Processes
Published in Pau Loke Show, Chien Wei Ooi, Tau Chuan Ling, Bioprocess Engineering, 2019
Kit Wayne Chew, Bervyn Qin Chyuan Tan, Jiang Chier Bong, Kevin Qi Chong Hwang, Pau Loke Show
Diafiltration is an extension of ultrafiltration and utilizes water during the concentration process. Diafiltration is used extensively in the pharmaceutical biotechnology fields, especially in the removal of low-molecular-weight components such as cations, anions, small proteins, or other anti-nutritional compounds. Diafiltration is also employed for concentrating the enzyme solution. The exceptional properties of diafiltration allow it to be utilized as an alternative process for dialysis and ion exchange. The membrane is also employed in the medical field for hemodialysis. The purification of nucleic acids can also be conducted by ultrafiltration membranes (Li et al., 2016).
Downstream Processing
Published in Sarfaraz K. Niazi, Disposable Bioprocessing Systems, 2016
Diafiltration is a membrane-based separation that is used to reduce, remove, or exchange salts and other small-molecule contaminant from a process liquid or dispersion. In batch diafiltration, the process fluid is typically diluted by a factor of two using “clean” liquid, brought back to the original concentration by filtration, and the whole process is repeated several times to achieve the required concentration contaminant. In continuous diafiltration, the “clean” liquid is added at the same rate as the permeate flow.
Recent developments in purification techniques and industrial applications for whey valorization: A review
Published in Chemical Engineering Communications, 2020
Navpreet Kaur, Poorva Sharma, Seema Jaimni, Bababode Adesegun Kehinde, Shubhneet Kaur
Baldasso et al. (2011) also purified the whey protein by using ultrafiltration together with discontinuous diafiltration (DF). The effects of volumetric-concentration factor (VCF), number of DF steps and the DF water volume were also checked. Results obtained from the study proved that UF is suitable for protein concentrate production and the yield of the protein concentrate obtained was more than 70% by weight. Discontinuous diafiltration was found to be more effective when performed with relatively smaller volumes and repeated several times.
Resource allocation strategies for protein purification operations
Published in IISE Transactions, 2020
Yasemin Limon, Ananth Krishnamurthy
In this article, we focus on the downstream protein purification operations. During a purification project, the scientist involved in the operations can use techniques such as virus inactivation, ultrafiltration, diafiltration, centrifugation and chromatography based on the characteristics of required protein and the impurities that need to be removed. Although purification scientists are highly skilled and receive training, the underlying uncertainty is significant, and purification outcomes could differ based on skill level of the scientist.