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Colorimetric Analysis
Published in Pradyot Patnaik, Handbook of Environmental Analysis, 2017
The first step in any colorimetric analysis is to prepare a standard calibration curve (i.e., a series of standard solutions of the analyte is made at a specific concentration range), which is then treated with the color-forming reagent, the absorbance or transmittance of which is then measured. The lowest calibration standard should be the minimum analyte concentration that would produce the least measurable absorbance or transmittance that should be the detection limit for the analyte. The highest calibration standard should be 10–15 times this concentration, and it must be within the linearity range. A plot is made between the absorbance or transmittance versus the concentration (or the microgram mass of the analyte). The concentration of the analyte in the sample is then read from the graph.
Smart Analytical Lab
Published in Shampa Sen, Leonid Datta, Sayak Mitra, Machine Learning and IoT, 2018
Subhrodeep Saha, Sourish Sen, Bharti Singh, Shampa Sen
Colorimetric analysis is used to determine the concentration of the analyte in a colored solution. The colorless analyte can be transformed into a visible color by the addition of a shading reagent. It is significant to both inorganic and natural mixes and might be used by means of enzymatic or nonenzymatic steps. This approach is normally used as a part of therapeutic labs and for mechanical purposes like examinations related to water treatment. The types of apparatus required for colorimetric examination are a colorimeter, an appropriate shading reagent, and some cuvettes. The method might be automated, for example, by using an auto analyzer or by flow infusion examination.
Production of the Roman Cement in Italy: characterization of a raw material used in Tuscany between 19th and 20th century and its comparison with a commercialized French stone material
Published in International Journal of Architectural Heritage, 2018
Emma Cantisani, Ambra Falabella, Fabio Fratini, Elena Pecchioni, Silvia Vettori, Fabrizio Antonelli, Marco Giamello, Marco Lezzerini
The colorimetric analysis was used for quantifying the color of the object under examination and to express it numerically; as a matter of fact, the perception of color can change depending on the observer, the light source illuminating the object, the background and the size of the object itself. In 1976, the color space L* a* b* was created by the CIE (“Commission Internationale de l’Eclairage”) to provide an objective measure of the color. L* indicates the brightness, a* and b* are the chromaticity coordinates and indicate color directions: + a* is the red direction, - a *is the green direction, + b* is the yellow direction, and - b* is the blue direction. When a and b increase, the color saturation increases.