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Dioscorea pentaphylla L.
Published in Parimelazhagan Thangaraj, Phytomedicine, 2020
S. Vivek, Y. Aron Santhosh Kumar, M. Palanisamy
The 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS+) decolorization assay involves the generation of the ABTS+ chromophore by the oxidation of the ABTS+ with potassium per sulfate. It is applicable for both hydrophilic and lipophilic compounds. The scavenging activity of the extract on the ABTS+ radical cation was measured at 734 nm (Giao et al. 2007). ABTS+ solution is prepared by following methods. An equal volume of 7 mM ABTS+ is mixed with 2.45 mM Potassium per sulphate. The mixture was allowed to stand in the dark place at room temperature for 12–16 hours. The sample is prepared by the addition of 1 ml of diluted ABTS+ with 10 µL of tuber extract in different concentration (50–250 µg/mL) 10 µL of ethanol is served as control. Ascorbic acid was used as the positive control. The absorbance was read at 734 nm after 6 minutes, and the percentage inhibitions were calculated. The inhibition was calculated according to the equation: I=Ao−A1/Ao×100,
Bioinspired Synthesis of Silver Nanoparticles: Characterisation, Mechanism and Applications
Published in Huiliang Cao, Silver Nanoparticles for Antibacterial Devices, 2017
Neeraj S. Thakur, Bharat P. Dwivedee, Uttam C. Banerjee, Jayeeta Bhaumik
ABTS is a colorimetric assay, in which the oxidation of ABTS [2,2´-azinobis (3-ethylbenzothiazoline-6-sulfonic acid)] with potassium persulfate generates ABTS radical cation; hydrogen-donating antioxidants reduce these ABTS cation radicals and decolourise the solution, which is measured at 734 nm (Mittal et al. 2014). The antioxidant activities of bioinspired AgNPs synthesised using C. sinensis was found to be 39% with an IC50 value 1.3 μg/mL, while a standard ascorbic acid revealed 59% (IC50 = 0.85 μg/mL) scavenging at the same concentration (Bhaumik et al. 2015). In DPPH colorimetric assay, the antioxidant donates hydrogen atom to the stable DPPH (1,1-diphenyl-2-picrylhydrazyl) radical, for which the DPPH solution is decolourised (Mittal et al. 2014). The antioxidant activity of bioinspired AgNPs was calculated to be 49% with an IC50 value of 5.0 μg/mL, while ascorbic acid showed 52% (IC50 = 4.8 μg/mL) scavenging at the same concentration (Bhaumik et al. 2015). Biosynthesised AgNPs can be valuable in therapeutics because of their considerable amount of antioxidant potential.
Microwave Synthetic Technology
Published in Banik Bimal Krishna, Bandyopadhyay Debasish, Advances in Microwave Chemistry, 2018
Biswa Mohan Sahoo, Bimal Krishna Banik, Jnyanaranjan Pa
Some triazolidine derivatives are prepared by using the microwave technique for their antimicrobial activity against the following microorganisms: Escherichia coli, Pseudomonas putida, Bacillus subtilis, Streptococcus lactis, Aspergillus niger and Candida albicans. Anti-oxidant activity screening assay is carried out by the ABTS method. Some compounds showed moderate or weak antimicrobial activity and promising antioxidant activity. The results of the study are given in Tables 11.6 and 11.7 [113].
Larvicidal activity of green synthesized zinc oxide nanoparticles from Carica papaya leaf extract
Published in Inorganic and Nano-Metal Chemistry, 2023
Manish Kumar Dwivedi, Suvashish Kumar Pandey, Prashant Kumar Singh
Several chronic health problems resulting from free radicals can be minimized by preventing the formation of free radicals. Research is underway to find novel sources of antioxidants of natural origin that are economically viable and harmless. ABTS assay is a radical scavenging assay that is used to evaluate the antioxidant activity of specific compounds. In the current study, concentration-dependent ABTS radical inhibition by CP-ZnONPs was observed with a maximum inhibition of 58.02 ± 0.21 at 100 mg/mL (Figure 9). The results depicted that the green synthesized CP-ZnONPs exhibited significant free radical scavenging activity.[42] Several studies have reported on the antioxidant properties of C. papaya leaves, unripe and ripe fruits, and seeds using DPPH and ABTS methods.[17] It has been reported that mature leaves had a higher percentage of radical scavenging properties than young leaves by DPPH and FRAP methods that might be due to higher flavonoid content.[62] Concentration-dependent antioxidant activity has also been reported from the leaf extracts of C. papaya.[63]
Detection of new phytochemical compounds from Vassobia breviflora (Sendtn.) Hunz: antioxidant, cytotoxic, and antibacterial activity of the hexane extract
Published in Journal of Toxicology and Environmental Health, Part A, 2023
Altevir Rossato Viana, Bruna Godoy Noro, Daniel Santos, Katianne Wolf, Yasmin Sudatti Das Neves, Rafael Noal Moresco, Aline Ferreira Ourique, Erico Marlon Moraes Flores, Cristiano Rodrigo Bohn Rhoden, Luciana Maria Fontanari Krause, Bruno Stefanello Vizzotto
The determination by ABTS involves the capture of the 2,2’-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) radical (ABTS). The capture of ABTS free radicals was measured as described by Re et al. (1999), where 5 ml ABTS stock solution (7 mmol/L) plus 88 μl potassium persulfate solution were used to generate the ABTS cation. The solution was left to stand at room temperature and in the dark for 16 hr. In a test tube, the hexane extract plus methanol (400 μl) was added to 2 ml ABTS radical solution. For control, the same reagents and volume were used, except for the hexane extract. A standard curve with 5 points (range 10–100 μg/ml) was prepared with a Trolox solution (1500 μmol/L), 400 μl was removed and 2 ml ABTS solution added at each point of the curve. After 6 min incubation, the absorbances were read at 734 nm (UV/VIS spectrophotometer – UV-1650 PC (Shimadzu®). The antioxidant activity was expressed through the amount of extract required to inhibit 50% of the radical (IC50).
The green-synthesized zinc oxide nanoparticle as a novel natural apoptosis inducer in human breast (MCF7 and MDA-MB231) and colon (HT-29) cancer cells
Published in Inorganic and Nano-Metal Chemistry, 2020
Seyed Hosein Boskabadi, Saeideh Zafar Balanezhad, Ali Neamati, Masoud Homayouni Tabrizi
The ABTS solution containing potassium persulfate (2.45 mM) and 2, 2′-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid ABTS (7 mM) was produced and incubated for 16 h at 25 °C in dark condition. Then, the mixture solutions containing (1:1) diluted solution (solution with 0.70 ± 0.02 absorbance at 734 nm) and solutions of different ZnONPs concentrations (500, 250, 125, and 62.5 µg/mL) was prepared. The mixture solutions were incubated for 1 h at 37 °C. Ultimately, the related absorbances were read at 734 nm. The free radical scavenging activity (FSA%) of samples were compared with Butylated hydroxyanisole (BHA). The protocol has been altered slightly according to Li et al. methods.[37] All experiments were performed in a triplicate manner (N = 3) in order to validate the findings.