China
Africa
Explore chapters and articles related to this topic
Laboratory tutorials
Published in Firdos Alam Khan, Biotechnology Fundamentals, 2018
Sectioning: The tissue is cut using a glass knife mounted on a microtome, or, for transmission electron microscopy, a diamond knife mounted on an ultramicrotome is used to cut 50 nm thick tissue sections that are mounted on a 3 mL diameter copper grid. The mounted sections are then treated with the appropriate stain. Frozen tissue embedded in a freezing medium is cut on a microtome in a cryostat.
in situ imaging of cell-wall polysaccharides in brown algae
Published in Bénédicte Charrier, Thomas Wichard, C.R.K. Reddy, Protocols for Macroalgae Research, 2018
Amandine Siméon, Delphine Duffieux, Cécile Hervé, Sophie Le Panse, Paul Knox, Thomas Torode
The use of a microtome allows generating thinner sections of equivalent size. We provide instructions for the use of a Leitz 1320 freezing microtome, but other systems can be used. Fill the sample holder with a Tissue Freezing Medium and place the sample. Coat the entire material with the Tissue Freezing Medium and wait until total freezing before sectioning. Sections of 60 µm are a good starting point for Laminariale stipes. Place sections in the fixative solution.
Molecular study of Chlorhexidine resistance in Methicillin Resistant Staphylococcus aureus
Published in Egyptian Journal of Basic and Applied Sciences, 2023
Salma O. Ghanim, Heba E. Eldegla, Marwa E. Sallam, Gamal M. Abdel-Fattah
MRSA cells were incubated with sub-inhibitory concentration of CHG (32 mg/L for the resistant strain and 1 mg/L for the susceptible strain) overnight at 37°C. Then the cells were collected and washed twice with PBS. For examination with a transmission electron microscope, the cells were centrifuged into a pellet, and their fixation was done with 2.5% of glutaraldehyde, then 1% Osmic acid. Dehydration of the samples was done by passing in an ethanol series (50%, 70%, 90% and 100%), followed by acetone. Then Spurr’s Epon resin was used for embedding of the samples. With the RMC PT-XL Ultra microtome, ultrathin sections were produced, then examined using JEOL JEM-2100EXII transmission electron microscope at 80 kV. The thickness of cell-wall was measured for one chlorhexidine-susceptible strain with MIC of 2 mg/L and one resistant strain with MIC of 64 mg/L because of the high cost of sample examination using transmission electron microscope [19].
Assessing the product quality of mango slices treated with osmotic and microwave drying by means of image, microstructural, and multivariate analyses
Published in Drying Technology, 2023
Sergio Jaubert-Garibay, Josué David Hernández-Varela, José Jorge Chanona-Pérez, Stefany Cárdenas-Pérez, Enrique J. Herrera-López, Gustavo F. Gutiérrez-López
Furthermore, a microstructural analysis of mango slices in T, L and O cuts at different processing conditions (F, OD, MD, and MOP) were observed with a confocal laser scanning microscope (CLSM) (LSM 880, Carl Zeiss, Germany). A small section of each cut and treatment from the surface of each sample with a disposable microtome blade was taken. Cellulose was examined by staining samples with calcofluor white M2R solution (Fluorescent Brightener 28, Sigma-Aldrich, St. Louis, Missouri, USA) at 0.1% for 2 min as well as using a diode laser (CW/pulsed) emitting at 405 nm. Carbohydrates were examined with Rhodamine B solution (Basic Violet 10, Sigma-Aldrich, St. Louis, Missouri, USA) at 0.01% for 2 min and observation was performed using the HeNe laser emitting at 540 nm.[27] The cellular size was estimated by directly measuring the cellular diameter using the ImageJ software, with at least 100 cells evaluated in each treatment. The images were captured at 20X magnification, in RGB color (1024 × 1024 pixels) and stored in TIFF format.
AFM observation of sea-island structure formed by second generation acrylic adhesive
Published in The Journal of Adhesion, 2021
Asuka Hayashi, Yu Sekiguchi, Chiaki Sato
Three types of samples were prepared for AFM analysis as shown in Figure 1. Sample A is the interface between the SGA and an adherend. In general, the interface cannot be observed with AFM. However, the interface was successfully observed with complete removal of the adherend from the SGA body by proper selection of the adherends. The inside of the interface was observed by scraping sample A with a knife (sample B). The sample preparation using a glass knife for a microtome or a razor blade could not provide enough surface flatness. Therefore, a diamond knife (DiATOME ultra 35°, Diatome, Biel, Switzerland) set in an ultra-microtome (UC6/FC6, Leica, Wetzlar, Germany) was used to obtain a surface flat enough to be observed with AFM. A film-shaped adherend was also sandwiched by SGA to observe the interface from the side, which was called sample C. The surface of sample C was also flattened using the diamond knife.