China
Africa
Explore chapters and articles related to this topic
Sturgeon (Acipenseridae)
Published in John A Plumb, Health Maintenance Of Cultured Fishes, 1994
Histologically, epithelial cells lining the straight intestine and spiral valve exhibited nuclear hypertrophy. Nuclei of some infected cells (up to one third of the cells in some fish) were five times larger than were noninfected cells. The infected cells continued to enlarge until they ruptured and released their contents into the gut lumen. Electron microscopy examination of affected tissues revealed large numbers of electron-dense hexagonal virions in the nuclei. The virions averaged 74 nm in diameter. Methyl green-pyronin stains of infected cells showed nuclear inclusions that contained DNA. Hedrick et al.1 tentatively placed the virus in the family Adenoviridae, based on DNA, morphology, and the absence of an envelope. Attempts to isolate the virus in white sturgeon spleen (WSS-1) and white sturgeon heart (WSH-1) cell cultures were not successful.
Comprehensive biological evaluation (DNA-binding, cleavage, and antimicrobial activity) of β-diketimine Schiff base ligands and their Cu(II) and Zn(II) complexes
Published in Journal of Coordination Chemistry, 2021
The groove-binding preference of the complexes was studied using DNA minor-groove-binder 4-6-diamidino-2-phenylindole (DAPI) and major-groove-binder methyl green (MG). The supercoiled pUC18 DNA was treated with DAPI or methyl green before adding the complexes. The electrophoresis results obtained with DAPI (lane 7 in Figure 5; lane 4 in Figure 6) are tracked by concurrent conversion of Form I to Form II/III in all complexes. However, no apparent inhibition of DNA damage was observed in the presence of major-groove-binder MG (lane 9 in Figure 4; lane 10 in Figure 6). This further strengthens the hypothesis of minor-groove preference for the complexes. These results are of significance as a majority of the oxidative cleavage reagents usually bind in the minor-groove mode [32].
Degradation of glyphosate herbicide by an electro-Fenton process using carbon felt cathode
Published in Environmental Technology, 2021
Manh Hai Tran, Hoai Chau Nguyen, Thanh Son Le, Viet Anh Dung Dang, The Ha Cao, Cao Khai Le, Trung-Dung Dang
As shown in Figure 9, the withdrawal of glyphosate follows an exponential function of time and the natural logarithm of Co/Ct ratio is linearly proportional to time. This behaviour confirms that the degradation of glyphosate by an electro-Fenton process belongs to a pseudo-first order reaction kinetics with the rate constant (kapp) of 0.063 min−1 and linear regression (R2) of about 0.996. Comparing to the kapp value of some dyes as direct orange 61 (0.301 min−1) [54], malachite green (0.39 min−1), crystal violet (0.36 min−1), methyl green (0.15 min−1), fast green (0.27 min−1) [61], the reaction rate constant of with glyphosate herbicide is more persistent than these dyes.
Synthesis and crystal structure of the first dinuclear zinc complex containing 1,4,7-triazacyclononane and biological properties of the protonated ligand
Published in Journal of Coordination Chemistry, 2018
Jing Qian, Wei-Wei Qiao, Jin-Lei Tian
The pBR322 DNA, CT-DNA, BSA, agarose gel, methyl green and SYBR green were purchased from Sigma Chemical Co. Other reagents of analytical grade were obtained from commercial suppliers and used directly without purification. Solvents used in this research were purified by standard procedures. Milli-Q water was used in all physical measurement experiments. Tris–HCl (Tris = tris(hydroxymethyl)aminomethane) buffer solution was prepared using deionized sonicated triple-distilled water. The stock solution of CT–DNA was prepared in 5 mM Tris–HCl/NaCl buffer at pH 7.4, the stock solution of the complex was prepared in DMF/H2O (1:10), and the stock solution of BSA was prepared by dissolving the solid BSA in 50 mM NaH2PO4/Na2HPO4 buffer at pH 7.4. All stock solutions were stored at 4 °C and used within 3 days.