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Thin-Layer Chromatography in Clinical Chemistry
Published in Bernard Fried, Joseph Sherma, Practical Thin-Layer Chromatography, 2017
Kovác and co-workers17 used the Hill reaction to analyze 18 herbicide residues in agricultural crops, foods, soil, and water. Pesticides were isolated from the matrix by liquid extraction. Concentrated extracts were cleaned up by column chromatography, and then the mixtures were separated in an NP system. The detection reagent was a mixture of a homogenate of bean leaves and of the redox indicator 2,6-dichloroindophenol. Dark blue inhibition zones were observed, which appeared on a pale yellow-green background during the exposure of the sprayed chromatographic plates to light. The dark blue zones disappeared again after some time; their lifetime was proportional to the amount of the herbicide in the zone. This chronometric method can be used for quantitation.
Plant Growth and Development Regulators and their Effect on the Functional State of Mitochondria
Published in Alexander V. Kutchin, Lyudmila N. Shishkina, Larissa I. Weisfeld, Gennady E. Zaikov, Ilya N. Kurochkin, Alexander N. Goloshchapov, Chemistry and Technology of Plant Substances, 2017
Irina V. Zhigacheva, Elena B. Burlakova
CHMS’s contribution to the thermostability of Hill reaction is the most important stage of the photolysis of water, which compensates the losses of electrons in chlorophyll and supports the restoration of NADP and ADP phosphorylation to ATP. At the same time, cryoprotective effect of CHMS and ES accompany a change in ratio of low and high molecular weight proteins and free amino acids in the phloem tissues of the shoots of grapes [87]. CHMS increases structural and functional stability of the plasma membrane of cells of wheat seedlings to the action of detergents. This reduces the loss of K+ ions and sensitivity of seedling roots to the action of the respiratory poisons.
Effects of varying PAHs-polluted soils on the morpho-anatomy and physiology of Bermuda grass
Published in Soil and Sediment Contamination: An International Journal, 2023
Pulchérie Matsodoum Nguemté, Guy Valérie Djumyom Wafo, Pierre François Djocgoue, Adrien Wanko
Chlorophyll is involved in the photosynthesis process, and therefore in plant nutrition. Inhibition of its production contributes to a physiological dysfunction that slows down plant development. Bohnert, Nelson, and Jensen (1995) stated that the availability of water for its biological roles as solvent and transport medium, as electron donor in the Hill reaction, and as evaporative coolant is often impaired by environmental conditions. Thus, due to the low water supply of Bermuda grass in polluted soils, water-deprived root cells rich in solutes (contained in cell organelles) will tend to conserve water by limiting their transport to the aerial parts and therefore the leaves. As a result, electrons will be supplied in small quantities by water following the latter’s deficit in photosystem II (P680) where photosynthesis begins. All of this combined with the low levels of chlorophyll “a” (the only pigment active in the photosynthesis process), the photosynthesis process will be impacted. In fact, drought stress is known to reduce photosynthesis by decreasing both leaf area and photosynthetic rate per unit leaf area (Basu et al., 2016). However, the significant evolution and development of the Bermuda grass under stress conditions suggests that this species has specific mechanisms that it activates to cope with the stress conditions set by PAHs. This result is in agreement with that of (Pérez-Hernández et al. 2013) for other plant species including Swietenia macrophylla King (Meliaceae). Furthermore, the anatomical and morphological impacts of PAHs on Bermuda grass are less significant than those of Amaranthus hybridus L. (Amaranthaceae) for which soil pollution by hydrocarbons is lethal at 4% (Omosun, Markson, and Mbansor 2008).