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Microfluidic Technologies for Cell Manipulation, Therapeutics, and Analysis
Published in Tuhin S. Santra, Microfluidics and Bio-MEMS, 2020
Amogh Kumar, Pallavi Shinde, Loganathan Mohan, Pallab Sinha Mahapatra, Tuhin S. Santra
The concentration of cells in the sample and the size of the microdroplets were adjusted accordingly to obtain the maximum number of droplets encapsulating single cells. Human liver hepatocellular carcinoma (HepG2) cells were used for experimental studies. The efficiency of trapping the droplets containing single cells reached up to 60%. Following cell capture, fluorescence studies were conducted to demonstrate the device efficiency in the single-cell analysis (Fig. 1.2). Thus, with further optimization, this device could show great promise in biological applications.
Lignin
Published in Antonio Paesano, Handbook of Sustainable Polymers for Additive Manufacturing, 2022
Zhang et al. (2020) selected colloidal lignin particles produced from softwood kraft lignin powder, crosslinked with Ca2+ ions, and combined them with cellulose nanofibers and alginate, and printed the resulting blend for soft TE. The blend resulted compatible with HepG2 cells that are human liver cells selected for studies of drug metabolism and toxicity.
Photoresponsive Polymers for Control of Cell Bioassay Systems
Published in Severian Dumitriu, Valentin Popa, Polymeric Biomaterials, 2020
Kimio Sumaru, Shinji Sugiura, Toshiyuki Takagi, Toshiyuki Kanamori
Meanwhile, the regulation of the culture environment by this cell-patterning method has much influence on the bioactivity of cultured cells. The HepG2 cells were seeded on a surface of a photoresponsive culture substrate which had been irradiated with a polka-dot pattern. HepG2 is a cell line derived from human hepatoma which is widely used in the research concerning the function of human liver. After incubation for 9 h, the cells floating above unirradiated area were removed. The patterned cells continued to grow and propagated within the irradiated area confined with a wall of PEG, forming evenly spaced uniform organoids in 5 days (Figure 20.19). Then balb/3T3 fibroblasts were introduced into the space among HepG2 organoids to form patterned co-culture system. Analysis on liver-specific gene expressions proved that the hepatic function of HepG2 such as cytochrome P450 activities and albumin production improved remarkably when cultured in such a patterned co-culture system compared with the conventional monoculture systems (Kikuchi et al. 2009). This experimental result suggested strongly that the patterned co-culture would increase the reliability of bioassay using liver-derived cells culture in vitro, which had been used as a means of high-throughput and low-cost drug screening (Norde and Gage 2004; Otsuka et al. 2004; Fukuda et al. 2006; O’Brien et al. 2006).
Preparation and characterization of dextran-zein-curcumin nanoconjugate for enhancement of curcumin bioactivity
Published in Journal of Biomaterials Science, Polymer Edition, 2023
N. T. S. Albogamy, Samia F. Aboushoushah, F. Aljoud, H. Organji, Nihal S. Elbialy
The cytotoxic effect of both free curcumin and DSZCNPs against human hepatoblastoma carcinoma (HepG2) cell line was assessed using MTT cell proliferation assay. The cells (HepG2, ATCC, USA) were obtained from the stem cell unit, king Fahd center for medical research, King Abdulaziz University (KAU), Jeddah, KSA. HepG2 cells (5 × 10^3 cells) were cultured in DEMAM (Dulbecco’s Modified Eagle’s Medium) supplemented with 10% FBS (Fetal bovine serum), streptomycin (1%), penicillin (1%) and 2 mM L-glutamine in 96-well plates and incubated for 24 h in 5% CO2-incubator. HepG2 cells were exposed to free curcumin and DSZCNPs with different concentrations (10–80 µg/ml), for 48 h. The percentage of viability of cells was assessed by using MTT assay where 20 µl of MTT added to each well, and they were incubated for four hours at 37 °C. The media was then discarded and 150 μl of MTT solvent was added. Finally, using a microplate reader, at 570 nm wavelength, the optical density (OD) was measured (Synergy HT, BioTek, Winooski, VT, USA).
pH-sensitive magnetic drug delivery system via layer-by-layer self-assembly of CS/PEG and its controlled release of DOX
Published in Journal of Biomaterials Science, Polymer Edition, 2020
Juan Wu, Xi Wang, Binglong Zhu, Qinting He, Fang Ren, Fei Tong, Wei Jiang, Xianghong He
Ferric chloride (FeCl3•6H2O), poly(acrylic acid) (PAA, Mn = 3000), polyethylene glycol (PEG) dicarboxylic acid (Mn = 600), 3-[4,5-dimethylthialzol-2-yl] − 2,5-diphenyltetrazolium bromide (MTT), dulbecco’s Modified Eagle medium (DMEM), fetal bovine serum (FBS), acridine orange base (AO) and ethidium bromide (EB) were purchased from Sigma-Aldrich. 2-hydroxypropytrimethyl ammonium chloride chitosan (CS) was supplied by Jinan Haidebei Marine Bioengineering Co. Ltd., Jinan, China. Sodium acetate (NaAc), sodium citrate (SC) and ethylene glycol (EG) were supplied by Aladdin. Doxorubicin (DOX) was purchased from Yuanye Biotechnology Co. Ltd., Shanghai, China. The human liver hepatocellular carcinoma cells (HepG2) were supplied by Chinese Academy of Sciences Cell Bank, Shanghai, China. All of the chemicals were analytical grade and used without further purification.
Production of triterpenoid compounds from Ganoderma lucidum spore powder using ultrasound-assisted extraction
Published in Preparative Biochemistry & Biotechnology, 2020
Shuang-Fei Shen, Li-Fang Zhu, Zijing Wu, Guangkun Wang, Zeeshan Ahmad, Ming-Wei Chang
HepG2 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Corning, NY, USA) and were supplemented with 10% fetal bovine serum (FBS, Gibco, Carlsbad, CA, USA) and 1% antibiotic (penicillin 100 U/mL, streptomycin 100 µg/mL). Cells were cultured in an incubator in 5% CO2 at 37 °C. The effect of triterpenoids on HepG2 cell viability was investigated using a CCK-8 assay. First, varying quantities of the triterpenoid extract were added to the DMEM medium at concentrations of 0.015, 0.03, 0.06, 0.1, and 0.2 mg/mL. HepG2 cells (grown in log phase) (100 µL) were introduced into 96-well plates and cultured for 24 hr to allow cell adherence. The original medium was removed and the medium containing the triterpenoid extract was replaced and cultured for 24 hr. The medium was mixed with CCK-8 at a ratio of 10:1. After 24 hr, the previous medium was removed and 100 µL of the mixture was added to each well and the plate was cultured in an incubator (37 °C). The absorbance was measured using a microplate reader at pre-determined time points. The cell survival rate was calculated as follows: