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Nano Resveratrol: A Promising Future Nanonutraceutical
Published in Bhupinder Singh, Minna Hakkarainen, Kamalinder K. Singh, NanoNutraceuticals, 2019
Chahinez Houacine, Kamalinder K. Singh
Chemopreventive activity of resveratrol was first discovered in 1997 (Jang et al., 1997). It is reported to exhibit antiproliferative properties against lymphoid and myeloid cancers, multiple myeloma, melanoma, squamous cell carcinoma, ovarian carcinoma, cervical carcinoma, and cancers of breast, prostate, stomach, colon, pancreas, thyroid, head, and neck. Its potential in colon cancer therapeutics has been shown through anticancer activity in HCT 116 cells, which is mediated by inhibiting PI3K/Akt signaling via upregulating BMP7 (Zheng et al., 2017). The antitumor potential of resveratrol has been attributed to its ability to bind to Cu2+ and cancer-involved G-quadruplexes in human melanoma cells (Platella et al., 2017).
Production of Life-Saving Drugs from Marine Sources
Published in Prasenjit Mondal, Ajay K. Dalai, Sustainable Utilization of Natural Resources, 2017
Salinosporamide A (18) is a potent proteasome inhibitor in phase II of clinical trial for the treatment of multiple myeloma. This marine NP is produced by marine bacteria found in ocean sediment, Salinispora tropica and Salinispora arenicola. A significantly potent proteasome inhibitor, salinosporamide A showed proteasomal chymotrypsin-like proteolytic inhibition activity with an IC50 value of 1.3 nM (Feling et al. 2003). Furthermore, it exhibited significant in vitro cytotoxicity against HCT-116 human colon carcinoma (IC50 11 ng/mL).
Fabrication and characterization of Ag nanoparticle-embedded κ-Carrageenan-Sodium alginate nanocomposite hydrogels with potential antibacterial and cytotoxic activities
Published in Journal of Biomaterials Science, Polymer Edition, 2023
Sonaimuthu Mohandoss, Sivarasan Ganesan, K. Velsankar, Sakkarapani Sudhahar, Fatemah H. Alkallas, Amira Ben Gouider Trabelsi, Fedor V. Kusmartsev, Huang-Mu Lo, Yong Rok Lee
The cytotoxicity of the κ-CGN-SA/AgNPs hydrogel nanocomposite samples was evaluated in against the HCT-116 and HeLa cell lines using the 3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Briefly, 12.5, 25.0, 37.5, 50.0, 62.5, 75.0, 87.5, and 100 μg/mL of the κ-CGN-SA/AgNPs hydrogel nanocomposite were UV-sterilized and incubated in 1 mL of Dulbecco’s modified Eagle medium at 37 °C for 48 h. Monolayers of HCT-116 and HeLa cells were seeded in culture flasks at 37 °C, 90% humidity, and 5.0% CO2/air. One milliliter of the conditioned media (κ-CGN-SA/AgNPs hydrogel nanocomposite incubated medium) was added in accordance with the ISO 10993-5 guidelines after 4 h of incubation, and the mixture was further incubated for 24 h at 37 °C. Then, 5 mg/mL MTT was added, and the mixture was incubated for another 4 h. As a control, the cells were maintained in the absence of the conditioned medium. The medium was then removed, DMSO was added, and the mixture was shaken for 30 min. The absorbance of the purple formazan crystals was measured at 600 nm using a plate reader (Biotek Power Wave XS, USA).
Unique enantiopure camphor-based neutral arene–ruthenium(II) complexes: DNA/BSA binding, kinetic and cytotoxic studies
Published in Journal of Coordination Chemistry, 2022
Milan M. Milutinović, Angelina Z. Caković, Dušan Ćoćić, Eduard Rais, Roland Schoch, Bojana Simović Marković, Nebojša Arsenijević, Vladislav Volarević, Snezana Jovanović-Stević, Jovana V. Bogojeski, René Wilhelm
Human breast cancer cell line MDA-MB and human lung carcinoma epithelial cell line A549 were purchased from American Type Culture Collection (ATCC, Manassas, USA). Human cancer colon cell line HCT-116 was kindly provided by Dr. Danijela Vignjevic (Institute Curie, Paris, France). Mouse melanoma cell line (B16F10) was purchased from American Type Culture Collection (ATCC, Manassas, USA). Mesenchymal stem cells (MSCs) were purchased from Invitrogen (Carlsbad, CA, USA). MDA-MB and HCT-116 cells were maintained in RPMI 1640 (Sigma Aldrich, Munich, Germany) supplemented with 10% fetal bovine serum, FBS (Sigma), penicillin G (100 IU/mL), streptomycin (100 μg/mL), and in a humidified atmosphere of 95% air/5% CO2 at 37 °C. The A549 cells, B16F10 cells, and MSCs were cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% FBS, 100 IU/mL penicillin G, and 100 μg/mL streptomycin (Sigma). Cell number and viability were determined by trypan blue staining. MDA-MB, HCT-116, A549 cells, B16F10 cells and MSCs in passage 3 were used throughout these experiments.