Fixed dose procedure

Acute Toxicity Testing by the Dermal Route

Published in Rhoda G. M. Wang, James B. Knaak, Howard I. Maibach, Health Risk Assessment, 2017

As noted previously, minimal lethal doses or approximate lethal doses may be quite adequate, if the test is conducted carefully. With these methods, administration of higher doses for the sole purpose of increasing numbers of deaths is not necessary. Instead, increasing the amount of biologic data (histopathology, clinical chemistry, etc.) from nonlethal doses may be more desirable.80,120 If we eliminate death as an endpoint altogether, both animal numbers and animal stress can be reduced. Methods such as the British Toxicology Society fixed-dose procedure (discussed previously) have been developed to accomplish this goal and are gaining in acceptance.

Antidiabetic and antihyperlipidemic effects of a methanolic extract of Mimosa pudica (Fabaceae) in diabetic rats

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Journal Information

Published in Egyptian Journal of Basic and Applied Sciences, 2019

Subramani Parasuraman, Teoh Huey Ching, Chong Hao Leong, Urmila Banik

Healthy, adult female SD rats were used for the experiment. The acute toxicity testing was performed by using the fixed-dose procedure. Overnight fasted rats were orally fed with the MEMP in increasing dose levels of 250, 500, 1000 and 2000 mg/kg body weight (n = 3 per dose), respectively. The animals were observed for their behavioral, neurological and autonomic profiles continuously for 24 h. After a period of 24 h, the animals were observed for 14 days for mortality. [22].

Fixed dose procedure

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Acute Toxicity Testing by the Dermal Route

Antidiabetic and antihyperlipidemic effects of a methanolic extract of Mimosa pudica (Fabaceae) in diabetic rats