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Published in Susma Bhattarai Gautam, Performance Assessment and Enrichment of Anaerobic Methane Oxidising Microbial Communities from Marine Sediments in Bioreactors, 2018
We have located the AOM site in coastal sediment from Marine Lake Grevelingen (the Netherlands) with the dominancy of ANME-3 (Bhattarai et al., 2017). Due to its similarity with in situ condition, i.e. ambient pressure conditions, enrichment of this anaerobic AOM community from the Marine Lake Grevelingen sediments in bioreactors will be easier and much efficient compared to enrichment of deep-sea sediments in laboratory scale bioreactors. In the current scenario of very limited knowledge on the ANME-3 ecophysiology, the enrichment of ANME-3 in bioreactors would be beneficial to understand the mechanism and physiology of ANME-3. Moreover, we have achieved enrichment of ANME-1 in the BTF and ANME-2 in the MBR. Based on the knowledge gained from this work, ANME could be enriched in different bioreactor configurations and thus enriched community can be further tested to understand its mechanisms, which seems promising advancement in the technical approach of AOM studies and way forward for its potential applications such as the removal of sulfate from wastewater using a cheap electron donor as methane. It is appealing to locate the ANME habitat in shallow marine environments, understand their key microbial processes and turnover and establish the links and roles played between different microbial communities when these sediments are transferred and cultivated in continuously operated bioreactors.
A colorimetric method for the determination of different functional flavonoids using 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and peroxidase
Published in Preparative Biochemistry and Biotechnology, 2019
Francisco R. Marín, Josefa Hernández-Ruiz, Marino B. Arnao
Flavonoids are phenolic compounds with a range of roles in biochemistry, plant physiology and ecophysiology.[1–5] They are involved in such important physiological phenomena as modulation of auxin transport,[6] pollen tube growth[7] and plant defenses, acting as chemical barriers,[8] among others. It is now widely accepted that the beneficial effects of fruit and vegetables in general health,[9,10] and also the prevention of specific dysfunction or diseases such as cardiovascular,[11–13] neurological,[14–16] metabolic (diabetic),[17,18] immunological,[19,20] and certain types of cancer,[21–24] can be ascribed to their bioactive components; also as anti-microbial agents against human pathogens[25–27] and in others situations.[28,29] Flavonoid content, as an extra added value for functional food, may be affected by food processing, as happens in citrus juices during squeezing.[30]