Concatemers

Concatemers

Elastin–Mimetic Protein Networks Derived from Chemically Crosslinked Synthetic Polypeptides

Published in Raphael M. Ottenbrite, Sung Wan Kim, Polymeric Drugs & Drug Delivery Systems, 2019

R. Andrew McMillan, Vincent P. Conticello

A synthetic DNA cassette was designed that encoded the repeat sequence Lys-25 (Figure 2). This DNA monomer was synthesized, sequenced, and self-ligated to afford a mixture of DNA concatamers. A 3,000 base pair concatameric gene was iolated from this mixture, which encoded a polymer of Lys-25 with a molecular mass of approximately 90 kD. Bacterial expression of the concatameric gene afforded a protein of the expected molecular mass that accumulates to high levels in E. coli (Figure 3).

Developing mitochondrial DNA field-compatible tests

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Journal Information

Published in Critical Reviews in Environmental Science and Technology, 2022

Bidhan C. Dhar, Christina E. Roche, Jay F. Levine

The RCA (Homs et al., 2008; Tang & Hyman, 2005) is an isothermal enzymatic procedure that could be used for mtDNA detection. In this approach, a short DNA or RNA primer is amplified to frame a long single stranded DNA or RNA using a circular DNA template, compatible buffer, dNTPs, and unique DNA or RNA polymerases. The product of RCA is a concatemer (a multiple of the same DNA in a long continuous DNA molecule) containing tens to many repeats in a final circular formation. The simplicity, of the DNA amplification system, has prompted it is use in biomedical research and nanobiotechnology and offers the promise of it being readily adaptable for detecting mtDNA in environmental samples.

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Elastin–Mimetic Protein Networks Derived from Chemically Crosslinked Synthetic Polypeptides

Developing mitochondrial DNA field-compatible tests