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Reduction and Fixation of Sacroiliac joint Dislocation by the Combined Use of S1 Pedicle Screws and an Iliac Rod
Published in Kai-Uwe Lewandrowski, Donald L. Wise, Debra J. Trantolo, Michael J. Yaszemski, Augustus A. White, Advances in Spinal Fusion, 2003
Kai-Uwe Lewandrowski, Donald L. Wise, Debra J. Trantolo, Michael J. Yaszemski, Augustus A. White
Bone marrow cells were flushed out of the cut ends of the femora of 8-week-old Balb/c mice (Jackson Labs, Bar Harbor, ME). The stromal population was separated from hematopoietic elements by serial passaging of adherent cells. They were then separated from bone marrow macrophages by maintenance of the cultures without the addition of factors that sustain the proliferation of macrophages. A colony of fibroblast-like cells, which responded to parathyroid hormone and produced alkaline phosphatase and colony-stimulating factor 1, was isolated with the use of a cloning ring. From this cloned cell population, one subclone was obtained by serial dilution with the use of 24 chamber plates and designated as D1. Subcloned cells were maintained in a-minimum essential medium with vitamins (Gibco, Grand Inland, NY), pH 7.36, containing 15% fetal bovine serum at 37°C in 5% carbon dioxide, and passaged with standard trypsin-ethylene-diaminetetraacetic acid techniques for a total of more than 50 passages. The cells were stored in a cryobiological cell bank until used. Transduction of D1 Cell with Traceable Genes
Preparation of graphene oxide nanoparticles and their derivatives: Evaluation of their antimicrobial and anti-proliferative activity against 3T3 cell line
Published in Journal of Dispersion Science and Technology, 2022
Mohammadamin Saedi, Vahid Shirshahi, Mehdi Mirzaii, Mohammad Nikbakht
3T3, a fibroblastic-like cell line, was purchased from Iran Cell Bank. The cells were maintained in a 75 cm2 plastic tissue culture flask containing Dulbecco’s modified eagle’s medium (DMEM), 10% (v/v) fetal bovine serum (FBS), and 1% (v/v) penicillin/streptomycin. The cells were incubated at 37 °C with 5% CO2. The cell culture medium was changed three times a week. After reaching the confluence, the cells were rinsed with PBS, and Trypsin-EDTA (0.25% Trypsin in 0.04 mM EDTA) was used to remove the adherent cells from the plate. Graphene solutions were precipitated at the bottom of cell culture plate wells for 48 hours. The cells with a density of 5 × 103 (cells/cm2) were cultured on the deposition of graphene material and incubated. They were treated in the presence of graphene precipitate at 24 and 72 hours. The MTT assay 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) was used to evaluate the cell viability and proliferation in vitro. The MTT solution of 100 mL with 0.5 mg/mL concentration was added to each well. After 4 hours, the medium containing MTT was removed, and 200 μL DMSO was added to dissolve the formazan crystals. The plate was incubated for 10 minutes and then maintained at room temperature for 15 minutes. Finally,100 μL of the final solution was transferred to the ELISA plate and read at 570 and 690 nm with a plate reader (Cytation 5, Biotek).
Metformin loaded injectable silk fibroin microsphere for the treatment of spinal cord injury
Published in Journal of Biomaterials Science, Polymer Edition, 2022
Qi Han, Tiantian Zheng, Linhui Zhang, Ningling Wu, Jiaqi Liang, Hong Wu, Guicai Li
Sprague Dawley (SD) pregnant rats with Specific Pathogen Free (SPF) grade 16 days gestation were provided by the Laboratory Animal Center of Nantong University. L929 mouse fibroblast cell line was purchased from Shanghai ATCC cell bank. Ethyl acetate was produced by American Anaqua Chemicals Supply Enke Chemical. Soluble freeze-dried silk fibroin was purchased from Jiangsu Suzhou Simeite Biotechnology Co., Ltd. Metformin, penicillin-streptomycin (PS), dopamine (DA) hydrochlonde, cytarabine, poly-lysine (PLL) and 4′,6-diamidino-2-phenylindole (DAPI) were all provided by Sigma-Aldrich, United States. Trypsin, fetal bovine serum (FBS) and Dulbecco’s Modified Eagle Medium (DMEM) high glucose basal medium were purchased from Gibco, USA. Chloral hydrate was produced by Sinopharm Chemical Reagent Co., Ltd. Phosphate buffered saline (PBS, pH7) was purchased from HyClone. Paraformaldehyde (PA) came from China National Pharmaceutical Group Chemical Reagent Co., Ltd. Immunofluorescence blocking solution, 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) kit, Actin-Tracker Green-488 were purchased from Shanghai Beyotime Biotechnology Co., Ltd.
Synthesis, X-ray structures, and biological activity of Zn(II) and cd(II) complexes with pyridine thiazolone derivatives
Published in Journal of Coordination Chemistry, 2021
Xunzhong Zou, Yanzhi Liao, Chaojie Yang, Ansheng Feng, Xiaoyun Xu, Huifeng Jiang, Yu Li
2-[4-(2-Pyridinyl)-2-thiazolyl]hydrazone (L1) and 2-[4-(3-pyridinyl)-2-thiazolyl]hydrazone (L2) were prepared according to previously reported literature [7]. Zinc p-toluenesulfonate hydrate and zinc hexafluorosilicate hydrate were purchased from Adama Reagent Co. Ltd. Cadmium p-toluenesulfonate hydrate was prepared by the reaction of p-toluenesulfonic acid and cadmium oxide. All other reagents and solvents were commercially available and employed as received or purified by standard methods prior to use. Four human cancer cell lines were kindly provided by Stem Cell Bank, Chinese Academy of Sciences. Elemental analyses were performed on a Vario EL analyzer (Elementar) and IR spectra on an Avatrar 330 FT-IR spectrometer (Thermo Nicolet) with potassium bromide pellets. The fluorescence (FL) spectra were collected on a Shimadzu RF5301PC photoluminescence spectrometer at room temperature. 1H NMR spectra were determined using a Bruker AVANCE-III 500. Biological activity tests were performed on a SpectraMax® ABS Absorbance Reader (Molecular Devices).