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Disposable Equipment in Advanced Aseptic Technology
Published in Maik W. Jornitz, Filtration and Purification in the Biopharmaceutical Industry, 2019
Maik W. Jornitz, Peter Makowenskyj
The final aseptic transfer of liquids and solid goods such as vial stoppers, syringe stoppers and plungers, monitors, and other items necessary for the aseptic process is certainly one of the most critical operations. Disposable aseptic transfer systems made of a single-use connector and a bag that contains the components to be transferred are either gamma sterilized by suppliers or by autoclaved at end users. In the latest case, autoclave sterilization needs to be validated by end users using spore strips of Bacillus stearothermophilus.
Production, purification, characterization, and applications of α-galactosidase from Bacillus flexus JS27 isolated from Manikaran hot springs
Published in Preparative Biochemistry & Biotechnology, 2023
Sonu Bhatia, Navneet Batra, Jagtar Singh
Various habitats have been explored for commercial production of novel α-galactosidases exhibiting properties like thermostability, pH stability, resistance toward proteases, high yield potential, variable substrate specificity, elevated catalytic efficiency, and immense synergistic capacity.[7] Thermostable enzymes from thermophilic microorganisms exhibit good tolerance to harsh industrial processes hence they are suitable at high processing temperatures in industries.[8–10] Thermostable α-galactosidases from extremophilic bacteria have been isolated and characterized from Thermotoga maritima, genus Geobacillus, Meiothermus taiwanensis, Bacillus stearothermophilus, Thermus thermophilus, etc.[11–13]
A study about the performance evaluation of medical waste sterilization plant in Van, Turkey
Published in Journal of the Chinese Institute of Engineers, 2022
The sterilization of medical waste from sterilization facilities is tested using biological indicators. For the bioindicator microbiological analysis of wastewater taken from the MWSP, the color change was checked after a period of 48 hours at a temperature of 30°C for Bacillus subtilis and at 55°C for Bacillus stearothermophilus. A yellow color seen in the bioindicator after incubation indicates insufficient sterilization, whereas purple indicates adequate sterilization. The bioindicator color seen in the test for this study after incubation was purple, denoting adequate sterilization was applied. Microbiological samples were kept in the oven for a period of 24 to 36 hours using the membrane filtration method. Total coliform, fecal coliform, and E. coli (Escherichia coli) bacterial species were not found to have vital level of activity. In the study by Küçükşahin, Özkaya, and Karadurmuş (2008) microbiological analysis of MWSP wastewater in Çorum also showed that total coliform and fecal coliform species were not found.
One-pot synthesis and characterization of Schiff base macrocyclic complexes as a potential bioactive core – a review
Published in Journal of Coordination Chemistry, 2021
J. P. Remiya, T. S. Sikha, B. Shyni
Synthesis, characterization and antimicrobial properties of some macrocyclic complexes of Fe(III), Co(III) and Cr(III) [Scheme S26, supplementary material] have been carried out by Sharma et al. [124]. These macrocyclic complexes were prepared through template condensation of ethylenediamine and 2,2-dihydroxyindane-1,3-dione using trivalent metals salts in methanol. The antimicrobial properties against two gram-positive (Bacillus subtilis, Bacillus stearothermophilus) and two gram-negative (Pseudomonas putida, Escherichia coli) bacteria were screened in comparison with chloramphenicol and streptomycin. [Co(C22H16N4O2)Cl]Cl2 exhibited a MIC of 4 µg/mL as shown by streptomycin towards P. putida. [Cr(C22H16N4O2)Br]Br2 and [Fe(C22H16N4O2)Br]Br2 exhibited MIC of 2 and 32 μg/mL against B. stearothermophilus and P. putida, respectively. Using MIC determination, [Co(C22H16N4O2)Cl]Cl2 was the most potent, whereas macrocyclic Fe(III) complexes showed low antibacterial activity.