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Reconstituted 2D Cell and Tissue Models
Published in Anthony J. Hickey, Sandro R.P. da Rocha, Pharmaceutical Inhalation Aerosol Technology, 2019
Nicole Schneider-Daum, Patrick Carius, Justus C. Horstmann, Claus-Michael Lehr
A549 cells are an adenocarcinoma cell line which is probably most frequently applied to emulate AT II-like pneumocyte function in vitro (Giard et al. 1973). The initially reported formation of lamellar bodies in A549 cells categorized them as AT II-like cells, whereas this functional characteristic is lost during longer culture times (Lieber et al. 1976; Speirs et al. 1991). Even though A549 cells show characteristic features of AT II cells with respect to drug transport and metabolism, their inability to form functional tight junctions disqualifies the A549 cell line from being used for transport studies because the discontinuous barrier shows no selectivity for low molecular weight compounds (Foster et al. 1998; Winton et al. 1998).
Characterizing the interaction between micro(nano)plastics and simulated body fluids and their impact on human lung epithelial cells
Published in Journal of Environmental Science and Health, Part A, 2023
Hasan Saygin, Ahu Soyocak, Asli Baysal, Ayse Mine Saridag
The cytotoxicity of the micro(nano)plastics has been examined in a few studies in which dermal, blood or colon cells, rather than A549 cells, were mainly used to determine the toxicological mechanisms.[9,20] To understand the cell responses, the zeta potentials and particle sizes of the plastic particles were investigated but other physicochemical characteristics were not appropriately addressed.[1,14,21–24] Toxicological endpoints are primarily examined based on mitochondrial activity; however, other toxicological endpoints (e.g., membrane lipids, and proteins) have been underestimated. The responses of inhalation- or respiratory-related cells have remained unexamined, although inhalation is one of the main exposure routes. In this field, A549 cells serve as a good model system as they reflect the human respiratory epithelium and are widely used to assess lung injury and the toxicology of lung tissue in vitro.[24] Moreover, many toxicological studies use the specific formation of pure polymers. However, plastic materials include additives that are added during the production of end products, and pure polymers exhibit different surface properties from the end products in terms of morphological attributes, size ranges, and aspect ratios.[18,20] These surface properties affect their fate.
PEG-modification on the endo-position of an antisense oligonucleotide increases tumor accumulation via the EPR effect
Published in Journal of Biomaterials Science, Polymer Edition, 2018
Kenji Hagiwara, Kana Kurihara, Masakazu Honma, Junichiro Yamamoto, Fumikazu Shinohara
A549 cells, derived from human lung adenocarcinoma, were obtained from American Type Culture Collection. The cells were maintained in Ham’s F-12K Medium (Gibco) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. The cells were harvested using trypsin treatment (Life Technologies) to make tumor-bearing models. The models were established by subcutaneous inoculation of 5 × 106 A549 cells in 0.05 mL PBS into the back of the mice. The tumor volume was calculated using the following formula: volume = width2 × length × 0.5. When the tumor volume reached approximately 100 mm3, mice were euthanized and used for each analysis.