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Multiple endocrine neoplasia type 1
Published in J. K. Cowell, Molecular Genetics of Cancer, 2003
To date, the MEN1 homologs from rat (Karges et al., 1999; Maruyama et al., 1999), zebrafish (Khodaei et al., 1999; Manickam et al., 2000), and drosophila (CG33266 in Flybase:http://flybase.bio.Indiana.edu:7081/annot/) have also been identified and characterized which share 96.7%, 67%, 50% identity with human menin, respectively. These homologs will definitely facilitate future studies in elucidating their functions and roles in tumorigenesis. Interestingly, from the full sequencing of yeast (Saccharomyces cerevisiae) and roundworm (Caenorhabditis elegans), no MEN1 homologs could be found.
An RNAi-mediated screen identifies novel targets for next-generation antiepileptic drugs based on increased expression of the homeostatic regulator pumilio
Published in Journal of Neurogenetics, 2018
Wei-Hsiang Lin, Miaomiao He, Yuen Ngan Fan, Richard A. Baines
Functional cluster analysis of 1166 dpum regulators was carried out using DAVID 6.8 software (the Database for Annotation, Visualization, and Integrated Discovery) (https://david.ncifcrf.gov) (Huang da, Sherman, & Lempicki, 2009a, 2009b). Sets of genes were uploaded using FLYBASE gene IDs. The p values for enrichment of genes in biological mechanisms were evaluated by Benjamini correction, and values less than 0.05 were considered significant. The molecular interaction networks of 101 dpum activity-dependent regulators were investigated using Cytoscape v. 3.5.1 software (http://www.cytoscape.org/) (Saito et al., 2012; Shannon et al., 2003). The networks of gene relationships were based on the Drosophila melanogaster gene annotation databases. Results were visualized using ClueGO v. 2.5.0 (Bindea et al., 2009), CluePedia v. 1.5.0 (Bindea, Galon, & Mlecnik, 2013), and Cytoscape plug-in apps. Selection criteria was at least three genes per node with a minimum of 4% of the associated genes from all uploaded genes in one node. The threshold of pathway network connectivity (Kappa score) was 0.4 and pathways with p values ≤.05 are shown.
Mnb/Dyrk1A orchestrates a transcriptional network at the transition from self-renewing neurogenic progenitors to postmitotic neuronal precursors
Published in Journal of Neurogenetics, 2018
Mirja N. Shaikh, Francisco J. Tejedor
All the fly stocks used in this study were derived from Dm and were raised at 25 °C on standard medium, except when particular temperature conditions were required (see below). All stocks have been described previously, and many of them are listed at FlyBase (http://flybase.bio.indiana.edu). The wt strains used were Berlin and Oregon-R. Fly stocks carrying mutations, transgenes and recombinant chromosomes were: ase1 (Campuzano et al., 1985); mnb1, (Tejedor et al., 1995); UAS-mnb RNAi (VDRC collection); pros-Gal4.C20, (F. Matsuzaki); elav-Gal4, (Bloomington Stock Center); c831-Gal4 (Colonques et al., 2011; Manseau et al., 1997); UAS-ase (Y.N. Yan), UAS-dpn (Wallace et al., 2000), UAS-mnb (Shaikh et al., 2016) and UAS-pros (F. Matsuzaki).
Antennae sense heat stress to inhibit mating and promote escaping in Drosophila females
Published in Journal of Neurogenetics, 2018
Yusuke Miwa, Masayuki Koganezawa, Daisuke Yamamoto
Flies were reared on cornmeal-yeast standard medium under a 12 h LD cycle at 25 °C, except for those carrying a dTrpA1 transgene, which were reared at 18 °C. The variants we used are described in the FlyBase database (http://flybase.org/). Wild-type Canton-S flies were used as the control. Gr28bMB03888 and Df(2L)Exel7031 are strains carrying a piggyBac-element insertion and deletion at the Gr28b locus, and were described in Xiang et al. (2010). The following fly lines were generous gifts from the indicated researchers: Gr28b.a-GAL4, Gr28b.b-GAL4, and Gr28b.c-GAL4 from H. Amrein (Thorne & Amrein, 2008); UAS-dTrpA1 from G. Miesenböck; UAS > STOP > dTrpA1myc and UAS > STOP > dTrpA1mCherry strains from B. Dickson (von Philipsborn et al., 2011); Otd-FLP from K. Asahina (Asahina et al., 2014); Ir93a-lexA from M. Gallio (Frank et al., 2015); Ir40a-GAL4 from R. Benton; 10XUAS > STOP > IVS-myr::GFP from B. Baker; UAS-TNT (UAS-TNT-G) and UAS-IMP-TNT (UAS-TNT-VA) from C. O’Kane (Sweeney, Broadie, Keane, Niemann, & O'Kane, 1995); and Gr5a-, Gr32a-, Gr33a-, Gr39b-, Gr43a-, Gr61a-, Gr64c-, Gr64e-, and Gr64f-GAL4s from V. Thoma (described in Weiss, Dahanukar, Kwon, Banerjee, & Carlson, 2011). A fly line with UAS > STOP > dTrpA1mCherry on chromosome 3 was obtained by mobilizing the transgene from the second chromosome to the third chromosome by means of Δ2–3 transposase-mediated P-element transposition (generated and provided by Y. Ishikawa). The following flies were obtained from the Bloomington Drosophila Stock Center: Gr28b.d-GAL4 (#57620, #58996), Gr28b.e-GAL4 (#57621), ey-FLP (#5618), 8XlexAop2-IVS-GAL80-WPRE (#32214), 10XUAS-IVS-mCD8::GFP (#32186), 10XUAS-IVS-myr::GFP (#32197), UAS-RedStinger (#8546), hs-FLP; 10XUAS > STOP > smGdP::HA, 10XUAS > STOP > smGdP::V5-THS-10XUAS > STOP > smGdP::FLAG (#64085), Gr28bMB03888 (#24190), Df(2L)Exel7031 (#7804), 20XUAS-6XGFP (#52261), and 20XUAS-IVS-CsChrimson.mVenus (#55136). P{Delta2-3}99B (#107–139) was obtained from KYOTO Stock Center (DGRC) at Kyoto Institute of Technology. VT11321-GAL4 (#207626) and VT44927-GAL4 (#207801) were obtained from the Vienna Drosophila Resource Center. All genotypes of examined flies are described in Supporting Information, Table S1.