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Advanced Nanoformulation Technologies in Cosmetic Science
Published in Heather A.E. Benson, Michael S. Roberts, Vânia Rodrigues Leite-Silva, Kenneth A. Walters, Cosmetic Formulation, 2019
Dynamic light scattering, and microscopic techniques such as cryo-transmission electron microscopy (Cryo-TEM) and atomic force microscopy (AFM) are used to characterize NPs for droplet sizes as well as polydispersity (Klang et al., 2012).
Liquid Crystals as Drug Delivery Systems for Skin Applications
Published in Andreia Ascenso, Sandra Simões, Helena Ribeiro, Carrier-Mediated Dermal Delivery, 2017
Liquid crystalline phases can be identified by several techniques, such as polarizing light microscopy, differential scanning calorimetry (DSC), small-angle X-ray scattering (SAXs), low- frequency dielectric spectroscopy, nuclear magnetic resonance (NRM), Raman scattering spectroscopy, cryo-transmission electron microscopy (Cryo-TEM), or cryo-field emission scanning electron microscopy (Cryo-FESEM) [4,9].
Assessment of stability of sulphated lactosyl archaeol archaeosomes for use as a vaccine adjuvant
Published in Journal of Liposome Research, 2021
Yimei Jia, Vandana Chandan, Bassel Akache, Hui Qian, Zygmunt J. Jakubek, Evguenii Vinogradov, Renu Dudani, Blair A. Harrison, Mohammad P. Jamshidi, Felicity C. Stark, Lise Deschatelets, Janelle Sauvageau, Dean Williams, Lakshmi Krishnan, Michael J. McCluskie
Archaeosomes preparation for cryo-transmission electron microscopy (TEM) was performed as previously described (Jia et al. 2019). Briefly, one 8 µL droplet of archaeosome aqueous sample at 0.5 mg/mL was placed on perforated carbon film supported by TEM grids after glow-discharged; excess solution was blotted and the TEM grid with specimen on was rapidly plunged into liquid ethane. The frozen TEM grids were then transferred to a storage box in liquid nitrogen before being transferred for TEM analysis. All TEM images were collected in JEOL 2200FS TEM with 200 kV accelerating voltage. An objective aperture and a 10 eV energy filter slit were used for enhancing the contrast of TEM bright field images. The low dose imaging mode was applied during imaging acquisition and the electron beam dose for each image was 2 e−/Å2.
Preparation of albendazole-loaded liposomes by supercritical carbon dioxide processing
Published in Artificial Cells, Nanomedicine, and Biotechnology, 2018
Faheem Maqbool, Peter M. Moyle, Madeleine S. A. Tan, Kristofer J. Thurecht, James R. Falconer
The crystallization process of a given solute can result in the formation of different polymorphic forms and this process is driven by conditions such as temperature, solvent properties, and stirring rate. Given scCO2 is a unique solvent, and a process controlled by temperature, a range of solid state characterization methods were used to assess polymorphic changes using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) analysis, diffraction scanning calorimetry (DSC) and thermogravimetric analysis (TGA) on the commercial and scCO2 processed ABZ. The result of the solid-state characterization provided direction for the manufacturing of liposomes; hence, SCF technology was further employed to manufacture liposomes. The manufactured liposomes were analysed for particle sizing and surface charge of liposomes. Cryo transmission electron microscopy (Cryo-TEM) was used to confirm morphology and particle size. In order to quantify the drug loading capacity, a method was developed using high performance liquid chromatography (HPLC). The time dependent drug release of the liposomes was performed at 37° C and compared with the control. This study provides original data on the solubility of ABZ in scCO2, changes in solid state due to scCO2 processing, production of liposomes using scCO2, drug loading capacity, particle size and morphology, and drug release using dialysis membranes, which is essential pre-formulation information and will be useful for subsequent research into ABZ loaded delivery system using SCF-assisted methods.
Application of an assay Cascade methodology for a deep preclinical characterization of polymeric nanoparticles as a treatment for gliomas
Published in Drug Delivery, 2018
Cristina Fornaguera, Miguel Ángel Lázaro, Pau Brugada-Vilà, Irene Porcar, Ingrid Morera, Marta Guerra-Rebollo, Cristina Garrido, Núria Rubio, Jerónimo Blanco, Anna Cascante, Salvador Borrós
Nanoparticle size and shape were further characterized by Cryo transmission electron microscopy (TEM) image analysis. As expected, nanoparticles showed smaller sizes than those obtained from DLS measurements and monomodal size distributions with mean size around 47 nm (Figure 2(B,C). In comparison with nanoparticles produced for previous studies, current nanoparticles were smaller, an advantageous trait in terms of BBB crossing and avoidance of detection by the reticuloendothelial system (RES) (Dobrovolskaia & McNeil, 2013; Tosi & Bortot, 2013). Nanoparticles had spherical morphology and smooth surface, as determined by CryoTEM (Figure 2(A)), as it had been predicted for nanosystems formed by this polymer.