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Africa
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Eukaryotic Mechanosensitive Ion Channels
Published in Tian-Le Xu, Long-Jun Wu, Nonclassical Ion Channels in the Nervous System, 2021
Evidence from different independent investigators demonstrated that Piezo channels are specialized force transducers activated by any physiological membrane tension generated by diverse mechanical forces, such as shear stress (blood flow), stiffness (bone) and stretch (lung) [3,24]. The most common in vitro mechanical stimulations are “stretch” and “poke” in combination with patch-clamp electrophysiology. In the “stretch” model, the membrane is stimulated using a high-speed pressure clamp, which results in highly reproducible pressure–response relationships. In the “poke” model, the membrane is indented with a blunt glass probe, leading to larger current amplitudes [24]. Piezo1, but not Piezo2, can also be activated by chemical activators, including Yoda1 [25], Jedi1/Jedi2 [26], and intrinsically by single strand RNAs [27]. Piezo channels can be inhibited by non-specific blockers, including ruthenium red, gadolinium ion, and the spider toxin GsMTx4 [22,28,29].
Probing the settlement signals of Amphibalanus amphitrite
Published in Biofouling, 2018
Mado Kotsiri, Maria Protopapa, Gesthimani-Myrto Roumelioti, Athena Economou-Amilli, Eleni K. Efthimiadou, Skarlatos G. Dedos
The effects of capsaicin, yoda1 and BITC on cyprid settlement and metamorphosis show that cyprids avoid noxious and harmful stimuli, as do other metazoan phyla (Im and Galko 2012). Yoda1, a compound identified as an activator of the mammalian mechanosensitive channel Piezo1 (Syeda et al. 2015), had an inhibitory effect on cyprid settlement and metamorphosis, but at very low concentrations it stimulated cyprid metamorphosis within 24 h (Table 1 and Figure S3). Although it needs to be proven that yoda1 actually binds to the barnacle orthologue of Piezo channel (Syeda et al. 2015), the prospect of mechanosensitive channel manipulation as a potential AF target merits further research.