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Therapeutic Efficacy of Black Pepper in Gastrointestinal Disorders
Published in Megh R. Goyal, Preeti Birwal, Durgesh Nandini Chauhan, Herbs, Spices, and Medicinal Plants for Human Gastrointestinal Disorders, 2023
Different herbs and spices including pepper consist of number of effective constituents, such as terpenoids, flavonoids, minerals, and phytoestrogens.3 Piperine is one of them with significant antioxidant activity that could decrease oxidative tissue damage, which was induced with excessive fatty acid food.72 In addition, it also reduces the amount of reactive thiobarbituric acid by maintaining the levels of different enzymes, such as glutathione, catalase, glutathione peroxidase, superoxide dismutase, and glutathione-S-transferase.72 In liver, piperine may enhance the biotransformation enzyme function.60
Functional Benefits of Ficus Hispida L.
Published in Hafiz Ansar Rasul Suleria, Megh R. Goyal, Health Benefits of Secondary Phytocompounds from Plant and Marine Sources, 2021
D. Suma, A. Vysakh, R. N. Raji, Ninan Jisha, M. S. Latha
Neuroprotective effect of methanolic extract of Ficus leaves was studied on β-amyloid induced oxidative stress and cognitive deficits in mice [27]. Reduction in cognitive behavior and memory deficit were shown by Ficus leaves. In brain, thiobarbituric acid reactive species level is reduced. During the study, antioxidant enzymes showed their increased activity. Alzheimer′s disease and age-associated memory disorders can be treated by applying these activities [32].
Plants from Brazil Used Against Snake Bites
Published in Mahendra Rai, Shandesh Bhattarai, Chistiane M. Feitosa, Wild Plants, 2020
Jocimar de Souza, Bruna Stramandinoli Deamatis, Fernanda Mayumi Ishii, Ingrid Francine Araújo de Oliveira, Gustavo Rodrigues Toledo Piza, Jorge Amaral Filho, Edson Hideaki Yoshida, José Carlos Cogo, Angela Faustino Jozala, Denise Grotto, Rauldenis Almeida Fonseca Santos, Yoko Oshima-Franco
The lipid peroxidation test evaluates thiobarbituric acid reactive substances (TBARS). These substances are products of lipid degradation, and data is presented in Table 7.7. A significant increase in the level of TBARS was observed in B. jararacussu group comparing T1 to T0. When the groups within T0 are compared, there is no statistical difference. However, a significant difference was found among venom group and all other groups within T1, which means that the venom affected the redox state and induced lipid peroxidation over time. On the other hand, TBARS levels decreased when ursolic and oleanolic acids were administered simultaneously to the venom, which is a very advantageous fact since the acids showed antioxidant protection on the lipid peroxidation induced by the venom.
Photoprotective effect of solid lipid nanoparticles of rutin against UVB radiation damage on skin biopsies and tissue-engineered skin
Published in Journal of Microencapsulation, 2022
Rodrigo Molina Martins, Silvia de Siqueira Martins, Gustavo Luis Ferreira Barbosa, Maria José Vieira Fonseca, Patrick J. Rochette, Véronique J. Moulin, Luis Alexandre Pedro de Freitas
Four hours after irradiation skin samples were frozen and underwent cryogenic grinding using the CryoMill system (Verder Scientific, Inc., Newtown, PA, USA). Crushed samples were then dispersed in 10 mM Tris, 150 mM NaCl, 10% glycerol, and 0.25% Triton X-100 at a ratio of 1:1. Protein content was determined using the BCA Protein Assay Kit. For the lipid peroxidation assay, 100 µL of each sample was added to 100 µL of 130 mM KCl/10 mM Tris-HCl (pH 7.4) buffer containing 10 µL of sodium citrate (200 mM) and 10 µL of ammonium iron sulphate following incubation at 37 °C for 30 min. Next, the thiobarbituric acid-reactive substance assay was carried out as previously described by Martins et al. (2020). Total metalloproteinase activity was determined using the SensoLyte 520 Generic MMP Assay Kit-Fluorimetric (AnaSpec, Fremont, CA, USA) according to the manufacturer’s protocol. The following metalloproteinase activities are detected using this kit: 1, 2, 7, 8, 9, 10, 12, 13, and 14.
Effects of sulfur dioxide, ozone, and ambient air pollution on lung histopathology, oxidative-stress biomarkers, and apoptosis-related gene expressions in rats
Published in Experimental Lung Research, 2022
Sorayya Kheirouri, Dariush Shanehbandi, Monireh Khordadmehr, Mohammad Alizadeh, Fateme Eskandari Vaezi, Razieh Musapour Sultan Abad, Mehran Mesgari-Abbasi
Interestingly, we noticed that in the sulfur dioxide group TAC was reduced and MDA and catalase activity was increased in comparison to the control group. But the reduction of TAC wasn’t statistically significant. MDA is one of the well-studied markers of the lipid peroxidation which is generated via peroxidation of polyunsaturated fatty acids in vivo and interacts with proteins.22 In the study of Meng et al., mice were exposed to 22, 56, and 112 mg/m3 of sulfur dioxide for 6 h/day for 7 days. Their findings showed a significant increase of thiobarbituric acid-reactive substances (MDA). Sulfur dioxide at low concentrations significantly increased SOD and GPx activities, whereas at high concentrations it significantly decreased them. Sulfur dioxide inhalation tended to decrease activities of catalase in lungs from mice of both sexes, whereas only the decrease of catalase activities caused by sulfur dioxide in lungs from male mice was statistically significant, at 112 mg/m3.40 In another study, mice were exposed to sulfur dioxide at 20 ppm (56 mg/m3). The results of the study indicated significant increase of TBARS level and significant decrease of SOD activity in lung tissue in comparison to the control group, while the catalase level differences were not significant.41
Doxorubicin-induced neurotoxicity is associated with acute alterations in synaptic plasticity, apoptosis, and lipid peroxidation
Published in Toxicology Mechanisms and Methods, 2019
Ahmad H. Alhowail, Jenna Bloemer, Mohammed Majrashi, Priyanka D. Pinky, Subhrajit Bhattacharya, Zhang Yongli, Dwipayan Bhattacharya, Matthew Eggert, Lauren Woodie, Manal A. Buabeid, Nathaniel Johnson, Alyssa Broadwater, Bruce Smith, Muralikrishnan Dhanasekaran, Robert D. Arnold, Vishnu Suppiramaniam
Hippocampal slices from rats were incubated in oxygenated submerged chambers containing vehicle-control aCSF or Dox containing aCSF. These slices were incubated for 6 h followed by weighing the tissue and homogenization with PBS and lysis buffer. The samples were sonicated (Qsonica, Newtown, CT) for 2–3 min. Then samples were centrifuged at 12 000 × g for 20 min at 4 °C. The supernatant was transferred into new centrifuge tubes. The proteins were quantified by Bradford Protein Assay before running the lipid peroxidation assay. A spectrophotometric method using thiobarbituric acid was used to assess lipid peroxidation. The index of lipid peroxidation was estimated by the formation of thiobarbituric acid-reactive substances (TBARS) at 532 nm. TBARS was normalized to total protein content as TBARS formed/mg protein (Ahuja et al. 2017).