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Bacille Calmette–Guerin and Prospects for New Vaccines against Tuberculosis
Published in Peter D O Davies, Stephen B Gordon, Geraint Davies, Clinical Tuberculosis, 2014
One other subunit vaccine candidate that is likely to enter into clinical testing in the not too distant future is the protein heparin binding hemagglutinin (HBHA) [87]. This antigen induces multifunctional CD4+ T cells in latent M. tuberculosis infection but not in active disease [88]. Purified HBHA, administered with a dimethyldioctadecylammonium bromide-monophosphoryl lipid A adjuvant, confers protection against M. tuberculosis challenge in mice [89]. This protein is currently in development as a potential vaccine candidate and also as a candidate for a diagnostic test.
Effect of PEG anchor in PEGylation of folate-modified cationic liposomes with PEG-derivatives on systemic siRNA delivery into the Tumor
Published in Journal of Drug Targeting, 2023
Min Tang, Sho Sakasai, Hiraku Onishi, Kumi Kawano, Yoshiyuki Hattori
Dimethyldioctadecylammonium bromide (DDAB, product name: DC-1-18) was obtained from Sogo Pharmaceutical Co., Ltd. (Tokyo, Japan). Methoxy-poly(ethyleneglycol)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine, sodium salt (mean molecular weight (MW) of PEG: 2000 and 5000, PEG2000-DSPE and PEG5000-DSPE, respectively), 1,2-distearoyl-rac-glycero-3-methylpolyoxyethylene (mean MW of PEG: 2000, PEG2000-DSG), poly(ethylene glycol) cholesteryl ether (mean MW of PEG: 1600, PEG1600-Chol), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), were obtained from NOF Co. Ltd. (Tokyo, Japan). Folate-PEG-DSPE (FA-PEG-DSPE) (mean MW of PEG: 2000 and 5000, FA-PEG2000-DSPE and FA-PEG5000-DSPE, respectively) was synthesized as described previously [24]. PEG3400-DSPE (mean MW of PEG: 3400) and FA-PEG3400-DSPE (mean MW of PEG: 3400) were obtained from Biochempeg Scientific Inc. (Watertown, MA, USA). Methoxypolyethylene glycol-chondroitin sulfate conjugate (mean MW of PEG: 2000, PEG2000-CS) was prepared as previously reported [20]. All other chemicals were of the highest grade available.
Effects of cationic lipids in cationic liposomes and disaccharides in the freeze-drying of siRNA lipoplexes on gene silencing in cells by reverse transfection
Published in Journal of Liposome Research, 2020
Yoshiyuki Hattori, Subin Hu, Hiraku Onishi
N-(2–(2-Hydroxyethylamino)ethyl)cholesteryl-3-carboxamide (OH-Chol) and cholesteryl (2-((2-hydroxyethyl)amino)ethyl)carbamate (OH-C-Chol) were synthesized as described previously (Hattori et al. 2015a). Cholesteryl (3-((2-hydroxyethyl)amino)propyl)carbamate hydroiodide (HAPC-Chol) was synthesized as described previously (Ding et al. 2008). 1,2-Dioleoyl-3-trimethylammonium-propane methyl sulphate salt (DOTAP) was obtained from Avanti Polar Lipids Inc. (Alabaster, AL). N,N-Dimethyl-N-octadecyloctadecan-1-aminium bromide (DC-1–18 or known as a dimethyldioctadecylammonium bromide, DDAB) and 11-((1,3-bis(dodecanoyloxy)-2-((dodecanoyloxy)methyl)propan-2-yl)amino)-N,N,N-trimethyl-11-oxoundecan-1-aminium bromide (TC-1–12) were obtained from Sogo Pharmaceutical Co., Ltd. (Tokyo, Japan). 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE, COATSOME ME-8181) was obtained from NOF Co. Ltd. (Tokyo, Japan). All other chemicals were of the highest grade available.
Comparison of antiproliferative effect of epigallocatechin gallate when loaded into cationic solid lipid nanoparticles against different cell lines
Published in Pharmaceutical Development and Technology, 2019
Amélia M. Silva, Carlos Martins-Gomes, Joana F. Fangueiro, Tatiana Andreani, Eliana B. Souto
Epigallocatechin gallate (EGCG, 98% purity, MW 458.375 g/mol, pKa 7.59–7.75; CAS Number: 989–51-5) and ascorbic acid (AA; MW 176.12 g/mol, CAS Number: 50–81-7) were acquired from Sigma-Aldrich (Sintra, Portugal). Dimethyldioctadecylammonium bromide (DDAB; MW 630.95 g/mol; CAS Number: 3700–67-2) was acquired from Avanti Polar Lipids (Alabama, USA). Softisan®100 (S100) was a free sample from Sasol Germany GmbH (Witten, Germany), Lipoid® S75, 75% soybean phosphatidylcholine was purchased from Lipoid GmbH (Ludwigshafen, Germany), Lutrol®F68 or Poloxamer 188 (P188) was a free sample from BASF (Ludwigshafen, Germany). Anhydrous glycerol was purchased from Acopharma (Barcelona, Spain). Ultra-purified water was obtained from a MiliQ Plus system (Millipore, Germany). All reagents were used without further treatment.