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Respiratory System
Published in Pritam S. Sahota, James A. Popp, Jerry F. Hardisty, Chirukandath Gopinath, Page R. Bouchard, Toxicologic Pathology, 2018
Tom P. McKevitt, David J. Lewis
Alveolar edema induced by alloxan was characterized ultrastructurally by marked disruption of both endothelium and epithelium, with swelling and loss of cells (Cottrell et al. 1967). One of the most utilized models of edema is alpha-naphthylthiourea (ANTU), which causes endothelial damage and gaps and which allow leakage of fibrin-rich fluid (Kehrer and Kacew 1985).
Methods in Experimental Pathology of Pulmonary Vasculature
Published in Joan Gil, Models of Lung Disease, 2020
Paul Davies, Daphne deMello, Lynne M. Reid
Inflammation causes edema by increasing permeability. For example, Michel et al. (1984) produced permeability edema in dogs by intravenous administration of alpha-naphthylthiourea (ANTU). Within 6 hr a dose of 27 mg/kg produced edema, and its development was monitored clinically. In addition, in this model (Michel et al., 1983) the effect on the amount of interstitial edema of three methods of fixation—airway instillation, vascular perfusion, and snap freezing—was compared between lobes processed by each of the three methods. In the experience of Michel et al. (1983) and that of others (Mazzone et al., 1978; Mazzone, 1981; Nicolaysen et al., 1975; Staub and Storey, 1962; Staub et al., 1967), snap freezing did not result in an increase in the amount of interstitial edema and also adequately preserved alveolar edema for visualization by light microscopy. Therefore this would be the method of choice for measuring the absolute amount of edema in the lung. Michel et al. (1983) found that fixation through the airways decreases the amount of alveolar edema, perhaps by washing it from the alveoli into the interstitium because of a pressure gradient in that direction. Perfusion fixation, on the other hand, was found to preserve alveolar fluid well but increased interstitial fluid. Compared with snap-frozen lungs, the absolute amount of edema in lungs fixed either by airway instillation or perfusion increased, but the proportions of interstitial edema around arteries and veins was not altered. Thus the latter methods could be effectively used for studying preferential edema accumulation.
Pinealectomy and melatonin administration in rats: their effects on pulmonary edema induced by α-naphthylthiourea
Published in Drug and Chemical Toxicology, 2023
Mohammed Raed Abdullah Al Gburi, Eyup Altinoz, Hulya Elbe, Melike Ozgul Onal, Umit Yilmaz, Nesibe Yilmaz, Melike Karayakali, Mehmet Demir
Alpha-naphthylthiourea (ANTU) is a toxic rodenticide that targets capillary epithelium in the lung tissue. Evidence suggests that ANTU elevates pulmonary vascular permeability in adult rats and leads to acute pulmonary edema and PE (Barton et al. 2000). Due to pulmonary toxicity and elevated pulmonary vascular permeability observed after ANTU administration, ANTU has been a popular pulmonary edema model in several morphological studies conducted with rats (Vivet et al. 1983). Intraperitoneal (i.p.) ANTU administration to rats in the standard empirical model induce dangerous, pulmonary edema, and PE levels within 4 h. The resulting edema could be completely treated within 24–48 h or the condition could gradually get worse and lead to death (Kaynar et al. 2013).