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Lectin
Published in Masahiko Mori, Histochemistry of the Salivary Glands, 2019
For histochemical detection of polysaccharides, including glycogen, the method of tissue fixation is important. In this respect, dehydrating fixatives such as alcohol, Carnoy’s solution, and the other fixatives are commonly employed for PAS staining. Takai et al.14 evaluated lectin bindings (11 kinds of lectins) in salivary glands of mice and rats with with 9 different fixatives, and Allison15 also described the binding of eight lectins in paraffin sections, using eight fixatives and frozen sections. Digestive organs and kidney were used.
Cysts and Tumours of the Bony Facial Skeleton
Published in John C Watkinson, Raymond W Clarke, Terry M Jones, Vinidh Paleri, Nicholas White, Tim Woolford, Head & Neck Surgery Plastic Surgery, 2018
Julia A. Woolgar, Gillian L. Hall
KCOT has a tendency to recur and the rate depends on the original treatment (overall recurrence rate, 20–25%). Local resection and enucleation with peripheral ostectomy and Carnoy’s solution are curative while enucleation with peripheral ostectomy, enucleation alone, marsupialization and enucleation with Carnoy’s solution are associated with increasing risk of recurrence (18%, 26%, 40% and 50% respectively in a recent systematic review).71
Recurrent odontogenic keratocyst with orbital invasion
Published in Orbit, 2022
Valerie Juniat, Alistair Varidel, James Badlani, James Nolan, Paul Sambrook, Dinesh Selva
Management choice for OKCs is balanced between reducing the risk of recurrence, which can result in the significant impacts as described below, and minimising morbidity to the patient caused by aggressive intervention in the first instance.5 A meta-analysis showed that the pooled weighted overall recurrence rate of OKCs for all different treatments was 16.6%.3 Conservative approaches such as enucleation alone and enucleation with curettage were found to have significantly higher recurrence rates than those involving enucleation with either Carnoy’s solution or liquid nitrogen cryotherapy.3 Carnoy’s solution is a cauterising agent used to create a zone of necrosis of approximately 1.5 mm deep when applied to a cystic bone cavity.6 A 10 ml solution contains 6 ml 95% ethanol, 3 ml chloroform, 1 ml glacial acetic acid and 1 g ferric chloride.6 Due to concerns regarding the safety of Carnoy’s, particularly the chloroform component, this is no longer available in this institution. A more recent study suggests peri-operative use of topical 5-fluorouracil (5-FU) has a role in reducing the risk of recurrence.7 However, 5-FU is unavailable for this indication at our unit.
The Protective Effects of Omega3 on Ubiquitination and Protamination of Rat Sperm after Bleomycin, Etoposide, and Cisplatin Treatment
Published in Nutrition and Cancer, 2018
Fatemeh Hashemi, Shahnaz Razavi, Farnaz Khadivi
Smears were fixed in Carnoy’s solution at 4 °C for 10 min. Slides were washed twice in phosphate-buffer saline (PBS), for 5 min at room temperature, then slides were incubated in acid-detergent solution pH =1.2 for 10 min at room temperature, washed twice in PBS. The slides incubated in blocking solution (3% BSA, 0.1% Tween 20, four times standard saline citrate solution) for 30 min and then incubated in mouse antiubiquitin antibody(Abcam, UK, 1:500), at 37 °C overnight. The slides washed in PBS three times for 10 min and incubated for 30 min in fluoresceinisothiocyanate (FITC)-conjugated antimouse (Abcam, 1:500). Images obtained through an Olympus fluorescent microscope (Olympus BX51, Japan) with the appropriate filters (460–470 nm) for each slide, at least 200 sperm were assessed. Assessment of ubiquitination was performed through Image J software. Sperm head with bright green considered positive for ubiquitination (27).
Cellular mechanisms regulating synthetic sex ratio distortion in the Anopheles gambiae germline
Published in Pathogens and Global Health, 2020
Roya Elaine Haghighat-Khah, Atashi Sharma, Mariana Reis Wunderlich, Giulia Morselli, Louise Anna Marston, Christopher Bamikole, Ann Hall, Nace Kranjc, Chrysanthi Taxiarchi, Igor Sharakhov, Roberto Galizi
Testes were dissected from late male Ag(PMB1) pupae and the wild-type A. coluzzii Mopti control on microscopic slides in hypotonic solution (0.075 M KCl). Tissues were fixed in modified Carnoy’s solution, covered in 50% propionic acid, immediately covered with a 22 × 22 mm coverslip, dipped in liquid nitrogen and serially dehydrated in 70%, 80% and 100% ethanol after removal of coverslips as previously described [8]. Sperm in testes from 4 males and at least 3 fields of view were counted per genotype. Counts represent elongated and flagellated sperm as it was not possible to differentiate between mature spermatozoa and elongating/flagellated intermediates.