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Distribution and Biological Functions of Pyruvate Carboxylase in Nature
Published in D. B. Keech, J. C. Wallace, Pyruvate Carboxylase, 2018
In yeast grown under conditions of biotin limitation, pyruvate carboxylase activity is low,156,572,573 and the cells contain substantial proportions of the apoenzyme.240,353,357,572 However, the biotin deficiency is not compensated for by an increased amount of pyruvate apocarboxylase.357 Similar results were noted for pyruvate carboxylase in Bacillus stearothermophilus grown under biotin-deficient conditions.161
Natural and physical preservative systems
Published in R. M. Baird, S. F. Bloomfield, Microbial quality assurance in cosmetics, toiletries and non-sterile Pharmaceuticals, 2017
Effects of combined pressure and temperature on Bacillus stearothermophilus spores showed that increasing pressure from 10 to 400 MPa gave the greatest reduction in numbers of surviving spores where the temperature was greater than 60°C (Taki et al. 1990).
Linear Regression
Published in Daryl S. Paulson, Applied Statistical Designs for the Researcher, 2003
A researcher is performing a steam-heat thermal-death curve calculation on a 106 microbial population of Bacillus stearothermophilus, where the steam sterilization temperature is 121°C. Generally, a log10 reexpression is used to linearize the microbial population. In log10 scale, 106 is 6. In this example, assume the microbial population is reduced 1 log10 for every 30 seconds of exposure to steam. This example is presented graphically in Fig. 5. b1 represents the slope of the regression line, which is the rise/run or tangent. This rise is negative because the value is decreasing over exposure time, so b0 represents the value of ŷ when x = 0, which is ŷ = 6 – 0.0333(0) = 6 in this example. For x = 60 seconds, ŷ = 6 – 0.0333(60) = 4.
Design and evaluation of in situ gel eye drops containing nanoparticles of Gemifloxacin Mesylate
Published in Drug Delivery, 2023
Vishwa J. Kalaria, S. Saisivam, Anas Alshishani, Jameel S. Aljariri Alhesan, Sumit Chakraborty, Mohamed Rahamathulla
The optimized formulation B3 in situ gel formulation containing Gemifloxacin Mesylate Nanoparticles was sterilized in an autoclave. During the sterilization process, a sterile filter paper strip impregnated with spores of Bacillus stearothermophilus as a biological indicator packed in an aluminum foil was also subjected to autoclave sterilization along with optimized formulation . The Biological indicator was transferred aseptically into petriplate containing nutrient agar medium and incubated at 37° C for 24 hrs. The Petriplate did not show any growth of Bacillus stearothermophilus. This proves that autoclave sterilization is proper. Apart from this, the sterilize optimized formulation was also subjected to test for sterility as per Indian Pharmacopeia (2018). The formulation passed the test for sterility proving that there is no microbial contamination.
Loop-mediated isothermal amplification methods for diagnosis of visceral leishmaniasis (kala-azar) – a systematic review
Published in Expert Review of Molecular Diagnostics, 2020
Gilberto Silva Nunes Bezerra, Walter Lins Barbosa Júnior, Amanda Virgínia Batista Vieira, Amanda Tavares Xavier, Manoel Sebastião Da Costa Lima Júnior, Edeneide Maria Xavier, Elis Dionísio Da Silva, Nilma Cintra Leal, Zulma Maria De Medeiros
Notomi et al. [25] developed the LAMP technology that amplifies a target DNA sequence with high sensitivity, specificity, and efficiency under isothermal conditions. The reaction employs a DNA polymerase (Bacillus stearothermophilus – Bst) with strand-displacement activity, two inner primers (FIP, BIP) and two outer primers (F3, B3) that recognize six different regions inside nucleic acid sequence (Figure 1). Two loop primers designed to anneal at the loop structure can increase reaction sensitivity and reduce detection time [26]. Also, the reaction can be monitored by simple visual detection of an insoluble salt formation or fluorescence [27].