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Methods for the Quantification of Histochemical Steroid Binding Assays*
Published in Louis P. Pertschuk, Sin Hang Lee, Localization of Putative Steroid Receptors, 2019
Even with the quantitative analysis, a variation of 10% in estimation of specimen composition would result in a significant change in the computed value. Two microscopists using computer-assisted microfluorometry obtain a mean relative fluorescent intensity of 175. One estimates the volume of tumor cells to be 20%, the other 30%. Calculations to correct the mean relative fluorescent intensity for tumor cell volume based on these estimates would result in a final classification of low to intermediate using the first estimate: (175 x 0.2) = 35; and of high to very high with the second: (175 x 0.3) = 52.5.
Cancer of the Breast
Published in P. Pertschuk Louis, Lee Sin Hang, Localization of Putative Steroid Receptors, 2018
Pietro Lampertico, Franca Stagni
The majority of investigators at the Milan meeting used normal mammary tissue or fibroadenoma as positive substrate controls and nontarget tissue as negative substrate controls. Antoci et al.13 preincubated tissue sections with unconjugated estrogens and obtained inhibition of ligand binding. On selected cases, the same group attempted semiquantification of positive tumors by limiting dilution studies, as suggested by Meijer et al.,14 and obtained satisfactory correlations with biochemical assays. A simple method of photometric evaluation might be obtained by studying the exposure time required for photography, or fluorescence quenching. A more sophisticated and precise approach using computer-assisted microfluorometry was reported by Pertschuk.15 Computerized morphometric analysis was also employed by Gambacorta16 in order to more precisely determine the distribution of cancer cells and stroma.
Evaluation Methods for Conditioned Hair
Published in E. Desmond Goddard, James V. Gruber, Principles of Polymer Science and Technology in Cosmetics and Personal Care, 1999
E. Desmond Goddard, James V. Gruber
The data show that the shampoo is capable of removing most of the deposited conditioner for which a film thickness of was calculated after the first treatment. However, a residual film is left after shampooing. Adsorption of anionic moieties during shampooing, which gradually desorb during rinsing, contributes to the reversal of the positive charge after conditioning. These two examples of the application of DEPA clearly demonstrate the usefulness of the method in characterizing the interactions between various cosmetic formulations, specifically conditioners and hair. Microfluorometry
Interference of altered plasma trace elements profile with hyperhomocysteinemia and oxidative stress damage to insulin secretion dysfunction in Psammomys obesus: focus on the selenium
Published in Archives of Physiology and Biochemistry, 2023
Asma Bouazza, Eric Fontaine, Xavier Leverve, Elhadj-Ahmed Koceir
Insulin resistance was estimated by the homeostasis model assessment of insulin resistance (HOMA-IR) method applied to experimental investigations. HOMA IR = fasting glucose (mmol/L) × fasting insulin (mU/L)/22.5 (Antunes et al. 2016). Plasma glycosylated haemoglobin (HbA1C) was measured by turbidimetry (Roche Diagnostic Systems, Basel, Switzerland). Plasma non-esterified fatty acids (NEFA) were determined by microfluorimetry. Plasma high-sensitive C-reactive protein (Hs-CRP) and plasma ferritin was assessed using immunoturbidimetric methods. Plasma iron was determined using the colorimetric methods on the automatic analyser (Synchron CX System, France). The fibrinogen was evaluated by the chronometric Von Clauss methods using hemostasis analyser ACL TOPTM (Biolabo, Maizy, France).
Modulating the cystic fibrosis transmembrane regulator and the development of new precision drugs
Published in Expert Review of Precision Medicine and Drug Development, 2018
Lionel Froux, Arnaud Billet, Frédéric Becq
The other promising approach is the use of patient induced pluripotent stem cells (iPSCs) to screen drug compounds in a more complex system [see review in 61]. Patient specific iPSCs could be isolated from blood samples [62] and differentiated in airway epithelium when cultured at air-liquid interface [63]. The cells obtained could be used with various tests such as compound screening by transepithelial current recording in Ussing chamber or flux measurement by microfluorimetry. Compared to classical bronchial primary epithelial cells, iPSCs cultures have the advantage to be undamaged (no inflammation or bacterial infection) and a limitless source of cells due to good replication properties [64]. Also, the cells could be genetically transformed by CRISPR-Cas9, becoming a strong tool for screening class I CFTR modulators [65,66]. Despite the promising contributions of iPSCs, the differentiation and culture of patient derived stem cells into an airway epithelium is still time consuming and expensive, limiting the use of such tool for drug screening. However, it could be a really powerful tool for precision medicine if the methods could be standardized and automatized in the future.
Differential expression and signaling of the human histamine H3 receptor isoforms of 445 and 365 amino acids expressed in human neuroblastoma SH-SY5Y cells
Published in Journal of Receptors and Signal Transduction, 2018
Gustavo Nieto-Alamilla, Juan Escamilla-Sánchez, María-Cristina López-Méndez, Anayansi Molina-Hernández, Agustín Guerrero-Hernández, José-Antonio Arias-Montaño
The microfluorimetry chambers were placed on a Zeiss LSM 700 Inverted Confocal Microscope, perfused with Hepes-buffered medium (HBM) at a rate 1 ml/min, and the depolarization-evoked changes in fluorescence were recorded every 1.5 s with an objective 63x. The composition of the HBM was (in mM): NaCl 139, KCl 3, MgCl2 1, CaCl2 2, D-glucose 11, Hepes 10; pH 7.4 with NaOH. For the high-K+ solution KCl (100 mM) was equimolarly substituted for NaCl.