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Bench testing for polymeric bioresorbable scaffolds
Published in Yoshinobu Onuma, Patrick W.J.C. Serruys, Bioresorbable Scaffolds, 2017
John A. Ormiston, Bruce Webber, Janarthanan Sathananthan, Pau Medrano-Gracia, Susann Beier, Mark W.I. Webster
The deployment of stents or scaffolds in a bifurcation phantom in a water bath and subsequent steps (e.g., proximal optimization, side-branch wire access, additional stenting) can be viewed live and recorded fluoroscopically [5,14]. The stent or scaffold can be temporarily removed from the water bath so a particular step can be photographed or a microCT image aquired. Photography through a microscope is very useful to record images of stents or scaffolds during a wide range of maneuvers. Multiple images are feasible and cost-effective. In the past we have imaged stent deployments through a borescope (pediatric endoscope) but this technique has been largely made redundant by microCT [15]. While microCT is limited by cost and imaging time required, it does allow unparalleled scaffold examination and images can be readily postprocessed with computer algorithms allowing slicing and advanced viewing such as fly-through [16]. The image reconstruction using gaming software distorts images and precludes accurate measurements. Intravascular ultrasound and optical coherence tomography are useful imaging tools for stents and scaffolds. For examining polymeric stent or scaffold coatings, we use environmental scanning electron microscopy which has very high resolution.
Proteus
Published in Dongyou Liu, Laboratory Models for Foodborne Infections, 2017
Paola Scavone, Victoria Iribarnegaray, Pablo Zunino
The difference between SEM and environmental scanning electron microscopy (ESEM) is that ESEM allows the examination of practically any specimen under any gaseous conditions and allows for obtaining images without prior specimen preparation. Holling et al. [55] used this tool to study the surface of catheters colonized by P. mirabilis biofilms. This tool permits the imaging of unprocessed, fully hydrated samples, which may provide much insight into the development of P. mirabilis biofilms.
Synthetic Polymers in Cosmetics
Published in E. Desmond Goddard, James V. Gruber, Principles of Polymer Science and Technology in Cosmetics and Personal Care, 1999
E. Desmond Goddard, James V. Gruber
Proper instrumentation to help understand the phenomena of drying is critical. The atomic force microscope (AFM) and environmental scanning electron microscope (ESEM) have been powerful tools useful in the investigation of latex film formation. Their application in hair fixative studies is overdue.
Alveolar bone remodeling after tooth extraction in irradiated mandible: An experimental study with canine model
Published in Ultrastructural Pathology, 2018
Venni Heinonen, Timo J. Ruotsalainen, Lauri Paavola, Jopi J. Mikkonen, Pekka Asikainen, Arto P. Koistinen, Arja M. Kullaa
The polished tissue blocks were acid-etched with 9% phosphoric acid for 40 s. Following etching, sections were immersed in sodium hypochlorite for 5 min and rinsed by hand in dH2O.17,19,18 The sections were placed in a desiccator overnight and thereafter the slices were coated by 20 nm gold layer for SEM imaging. The specimens were examined and photographed using either a XL30 ESEM TMP (Fei Co, Eindhoven, The Netherlands) or Zeiss Sigma HD VP (Carl Zeiss GmbH, Oberkochen, Germany) scanning electron microscope operated at 15 KV.
Effects of irradiation in the mandibular bone loaded with dental implants. An experimental study with a canine model
Published in Ultrastructural Pathology, 2021
Sridhar Reddy Padala, Pekka Asikainen, Timo Ruotsalainen, Jopi JW Mikkonen, Tuomo S Silvast, Arto P. Koistinen, Engelbert A.J.M. Schulten, Chris M. Ten Bruggenkate, Arja M. Kullaa
The polished tissue blocks were acid-etched with 9% phosphoric acid for 60 seconds. Following etching, sections were immersed in sodium hypochlorite for 3 min and rinsed in distilled water.22–24 The sections were placed in a desiccator overnight and thereafter the slices were coated by a thin 30–40 nm gold layer for SEM imaging. The specimens were examined and photographed using an XL30 ESEM TMP (Fei Co, Eindhoven, The Netherlands) or Zeiss Sigma HD VP (Carl Zeiss GmbH, Oberkochen, Germany) scanning electron microscope operated at 15 kV.
Polymeric nanodroplets: an emerging trend in gaseous delivery system
Published in Journal of Drug Targeting, 2019
In this technique, the electrons liberated by NDs are measured by the wet Scanning Transmission Electron Microscopy (wet-STEM) which acts as a detector for the Environmental Scanning Electron Microscope (ESEM). Monte Carlo simulation results are used as the experimental results for the determination of shape and contact angle of NDs. The combination of static contact angle of theoretical aspect and conversion of droplet size into nanoscale leads to the change in property and development of NDs [54].