Development of chlorine-induced lung injury in the anesthetized, spontaneously breathing pig
Published in Toxicology Mechanisms and Methods, 2021
Rachel Watkins, Rosi Perrott, Simon Bate, Philippa Auton, Sarah Watts, Alexander Stoll, Stephen Rutter, Bronwen Jugg
The right internal jugular vein was cannulated with an 8 F Desivalve introducer sheath (Vygon, Swindon, UK) and the right common carotid artery cannulated with a 7 F Desivalve introducer sheath using the Seldinger technique. A balloon tipped flow-directed cannula (744MF75 Swan Ganz, Edwards Life Sciences Ltd, Newbury, UK) was placed in each of the right vessels to enable measurement of continuous real time arterial and venous oxygen saturation via Vigilance II (Vigilance Volumetrics CEDV, Edwards Lifesciences, US). As soon as intravenous access was available an infusion of Vetivex 11 (Hartmann’s solution) (2.0 ml·kg−1·h−1) was delivered to replace insensible fluid losses. Finally, a Bonanno catheter was introduced into the bladder via an open cystotomy.