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A storm breaks: The White Paper
Published in John Marks, The NHS: Beginning, Middle and End?, 2017
In spite of the crisis in the health service I still had to attend to other matters affecting the BMA and my personal life. On 9 February John Havard and I went to Utrecht, along with our wives, for a meeting of European doctors. I got home on Saturday and the following day I drove to a meeting of the BMA’s Welsh Council, which met in Llandridnod Wells, in the centre of the country. Transport between the north and south of Wales is very difficult at the weekend, but no town in South Wales was acceptable to the northerners as a meeting place and vice versa, and so for many years the meeting had been held in Shrewsbury which has reasonably good access to both parts of the principality. After a while someone realised that Shrewsbury was in England, and demanded that the meeting be moved, and Llandridnod Wells, the pole of inaccessibility, was the best compromise that could be reached. The following day I briefed the other ‘Heads of Professions’ about our problems and a few days later I attended a dinner at the Royal College of Nursing, where further networking took place. Subsequently, I had a private and very unofficial meeting with Robin Cook, and I also managed to put in an appearance at a dinner organised by The British Life Assurance Trusts (BLAT).
Analysis of microbial communities of ocular prostheses and anophthalmic sockets using 16S rRNA gene sequencing
Published in Biofouling, 2023
L. R Makrakis, V. C Oliveira, E. S Santos, C Nascimento, E Watanabe, A. B Ribeiro, C. H Silva-Lovato
The data were processed, firstly, in Illumina BaseSpace Sequence Hub using the default quality control parameters. Briefly, low-quality sequences, short fragments, and sequences derived from polyclonal amplification were removed. Reads were trimmed, aligned, and assembled into a single contig at a minimum sequence identity of 97%. The most extended sequence was picked as the cluster representative, and a BLAT search was then performed against the Ribosomal Database Project (RDP). OTUs (operational taxonomic units) were classified using a set containing the complete Greengenes database supplemented with eukaryotic sequences from the Silva databases. A minimum alignment length cutoff of 200 bp, minimum read coverage of 98%, and an e-value cutoff of 1 × 10−5 were applied in the analysis.
Expression of cytochrome P450 regulators in cynomolgus macaque
Published in Xenobiotica, 2018
Yasuhiro Uno, Hiroshi Yamazaki
Sequence data were analyzed using DNASIS Pro ver2.09 (Hitachi Software, Tokyo, Japan), the Genetyx system (Software Development, Tokyo, Japan), or Sequencher (Gene Codes Corporation, Ann Arbor, MI). The ClustalW program was used for multiple alignment of amino acid sequences. The neighbor-joining method (Saitou & Nei, 1987) incorporated in DNASIS Pro was used for phylogenetic analysis. A homology search was carried out by BLAST (National Center for Biotechnology Information), and the human and cynomolgus macaque genome data was analyzed using BLAT (UCSC Genome Bioinformatics). Amino acid sequences used were from GenBank, including human AhR (NP_001612), CAR (NP_001070950), and PXR (NP_003880); cynomolgus AhR (EHH52292), CAR (NP_001306471), and PXR (EHH51056); pig AhR (NP_001289955), CAR (NP_001033085), and PXR (NP_001033094); dog AhR (XP_532485), CAR (XP_005640928), and PXR (AAM10632); rat Ahr (NP_037281), Car (NP_075230), and Pxr (NP_443212); and mouse Ahr (NP_038492), Car (NP_033933), and Pxr (NP_035066).
A comprehensive analysis of six forms of cytochrome P450 2C (CYP2C) in pigs
Published in Xenobiotica, 2022
Yasuhiro Uno, Saho Morikuni, Mitsuya Shiraishi, Atsushi Asano, Hiroaki Kawaguchi, Norie Murayama, Hiroshi Yamazaki
The Genetyx system (Software Development, Tokyo, Japan) was used to analyse sequence data, including the ClustalW program for multiple alignment of amino acid sequences and the neighbour-joining method for the phylogenetic tree. BLAST (National Centre for Biotechnology Information) was used for the homology search using the amino acid sequences from GenBank or the present study. To determine gene and genome structure, the genome data were analysed by BLAT (UCSC Genome Bioinformatics) and Sequence Viewer (National Centre for Biotechnology Information) using the cDNA sequences from GenBank or the present study.