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Ad Hoc Integrated Library Functions Currently Used by Medical Libraries
Published in Helis Miido, The Integrated Medical Library, 2020
Interlibrary loan requests in the ORDER database are processed in the library, using the computer-assigned accession number. For example, if the last accession number processed was 5000, then processing begins the next day with 5001 up to the last reference typed. A listing (referred to as LIST) is first generated alphabetically by journal title, and run through a computer program which compares the title against holdings (including years of coverage) of select libraries. The name of the library where the item is available is inserted in LIST, together with the address, code and telephone number. The code of the lending library is entered by library staff in the records to be processed. (The computer program does not automatically update each record with the code of the lending library; this has to be done manually for each record.) Listings are produced (or files created for uploading to the British Lending Library) by lending source. Each interlibrary loan references the computer-assigned accession number as well as the requestor, so that when the item is received, it can be quickly retrieved from ORDER, logged in as received, and forwarded to the requestor.
DICOM – Medical Image Communication
Published in Arvind Kumar Bansal, Javed Iqbal Khan, S. Kaisar Alam, Introduction to Computational Health Informatics, 2019
Arvind Kumar Bansal, Javed Iqbal Khan, S. Kaisar Alam
A single patient may have multiple studies. The study-level attributes are associated with the study IE of the composite IODs. Study IEs are modality-independent. Date, time, accession-number, study-ID and study-IOD are required attributes. Study-instance UID is a unique attribute.
Head and Neck Pathology
Published in John C Watkinson, Raymond W Clarke, Terry M Jones, Vinidh Paleri, Nicholas White, Tim Woolford, Head & Neck Surgery Plastic Surgery, 2018
Ram Moorthy, Adrian T. Warfield, Max Robinson
Upon receipt in the laboratory, the specimen identity is corroborated and a unique accession number is allocated. Following an appropriate period of fixation (24–48 hours), either the pathologist or senior biomedical scientist staff will describe the specimen macroscopically, and dissect and submit representative tissue slices for examination. These slices are inserted into proprietary sealable cassettes appropriately labelled.
Genetic analysis of 23 Y-STR loci in the Va population from Yunnan Province, Southwest China
Published in Annals of Human Biology, 2023
Jing Yuan, Lei Huang, Yuan Yin, Xiufeng Zhang
Genomic DNA was extracted from FTA cards with the Chelex-100 method (Walsh et al. 1991). PCR for 23 Y-STR loci was carried out on a GeneAmp PCR system 9700 (Applied Biosystems, USA) using the PowerPlex Y23 (Promega Corporation) PCR Amplification kit according to the protocol described by the PPY23 and using 10.0 μl reaction volume for each sample, which contains 2.0 μl PCR Master Mix, 1.0 μl Primer Pair Mix, 6.0 μl ddH2O, and 1.0 μl template DNA. PCR conditions had the following steps: pre-denatured 96 °C for 2 min, followed by 30 cycles of 94 °C for 10 s, 61 °C for 1 min, 72 °C for 30 s, a final extension hold at 60 °C for 20 min, and a final soak at 4 °C. The amplified products were separated on an ABI 3130XL Genetic Analyser (Applied Biosystems, USA), and subsequently analysed by GeneMapper ID-X v.1.5 software (Thermo Fisher Scientific, Waltham, MA, USA) in comparison with the allelic ladders provided in the kit. The DNA typing and assignment of nomenclature were based on the ISFG recommendations (Bar et al. 1997; Lincoln 1997). 2800 M Control DNA and Nuclease-Free Water were used as positive and negative controls, respectively. Our laboratory has participated and passed the YHRD quality control. The population accession number is YA005788.
Comparing CAR and TCR engineered T cell performance as a function of tumor cell exposure
Published in OncoImmunology, 2022
Tassilo L. A. Wachsmann, Anne K. Wouters, Dennis F. G. Remst, Renate S. Hagedoorn, Miranda H. Meeuwsen, Eline van Diest, Jeanette Leusen, Jürgen Kuball, J. H. Frederik Falkenburg, Mirjam H. M. Heemskerk
The 1E9 TCR, described in Ref.14, was cysteine modified, constant domain murinized, codon optimized and cloned into the pMP71 flex vector. 1E9 TCR is a HLA-A*02:01-restricted, high-avidity TCR isolated from an individual that was negative for HLA-A*02:01. CAR constructs were cloned into pLZRS-P2A-dNGFR vectors. The FMC63-28z CAR sequence was taken from Ref. 15, accession number HM852952. For ofatumumab- and rituximab-based CARs, amino acid sequences for heavy and light chain antigen binding domains were extracted from patents no. US 7,850,962 B2 and no. US 5,843,439, respectively. scFvs were generated in a Vl-Vh configuration using a 4GS linker, and a CD8a leader sequence was cloned upstream. To generate 28z CARs, CD28 hinge, transmembrane, and signaling domains together with the CD3z domain were fused to Ofa or RTX scFvs using overlapping primer PCR analogous to the FMC63-28z construct. For BBz CARs, sequences encoding for Ofa or RTX scFvs were fused to CD8a hinge and transmembrane domains, 4–1BB costimulatory, and CD3z signaling domain, following the design of clinically used FMC63-BBz CAR.16
Comparing membrane and spacer biofouling by Gram-negative Pseudomonas aeruginosa and Gram-positive Anoxybacillus sp. in forward osmosis
Published in Biofouling, 2019
Anne Bogler, Douglas Rice, Francois Perreault, Edo Bar-Zeev
Cultures of P. aeruginosa (Gram-negative) and an Anoxybacillus sp. (Gram-positive) were used as model biofilm strains for all the experiments. P. aeruginosa was purchased from ATCC biosource center (ATCC47085D-5) as a well-studied biofilm strain, which is commonly used as a model bacterium for membrane biofouling in wastewater systems. Anoxybacillus sp. was isolated from effluent of a membrane bioreactor (MBR) treating a mixture of domestic and olive mill wastewater, since limited work has been done with Gram-positive bacteria that form biofouling in membrane based systems. Isolation of the Anoxybacillus sp. was done by plating a diluted MBR effluent sample on a Luria–Bertani broth (LB) agar (Becton, Dickinson and Company, Franklin Lakes, NJ, USA). A single colony was picked following overnight (∼12 h) incubation and re-cultured in LB for ∼12 h (this cycle was repeated three times). DNA from this MBR-effluent isolated sample was then extracted using a phenol chloroform approach according to Bar-Zeev et al. (2008). The V1–V3 regions of the bacterial 16S rRNA were amplified with a 28 F and 519 R primer pair according to Campbell and Kirchman (2013). Anoxybacillus sp. (most likely A. gonensis) was identified with 99% similarity by DNA Sanger-sequencing (Hy-labs, Rehovot, Israel). The accession number and sequence data are included in the Supplemental material.