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Effect of tomato juice on the sperm quality of mice exposed to tertiary cigarette smoke
Published in Ade Gafar Abdullah, Isma Widiaty, Cep Ubad Abdullah, Medical Technology and Environmental Health, 2020
A.R. Furqaani, A.K. Sari, R. Ekowati, L.H. Siswanti, A. Triamullah, T. Sugiartini
This research consisted of an in vivo laboratory experimental study with a completely randomized design. Seventeen adult male mice were randomly divided into three groups, the control group and two treatment groups. Treatment group 1 (T1) was exposed to tertiary cigarette smoke for 14 days, and treatment group 2 (T2) was exposed to tertiary cigarette smoke and given 0.64 mL of tomato juice for 14 days. After completion of treatment, the animals were sacrificed by cervical dislocation. Spermatozoa were taken from the cauda epididymis. The parameters measured in sperm examination included number, morphology, and motility of spermatozoa. At the time of surgery, testes were also isolated in order to identify cells’ density in testicular tissue. Data are presented in mean ± SD (standard deviation). Each parameter was analyzed using an ANOVA one-way test and the Tukey post hoc test at the 95% confidence interval (α = 0.05). This study was reviewed and approved by the Ethical Committee of the Medical Faculty at the Islamic University of Bandung with approval number 058/Komite Etik.FK/IV/2019.
Experimental Studies
Published in M. Venkataswamy Reddy, Statistical Methods in Psychiatry Research and SPSS, 2019
The completely randomized design (CRD) is the simplest of all the experimental designs based on the principles of replication and randomization. Let us suppose that there are k treatments and the ith treatment is being replicated ni times. In CRD, the whole of the experimental units are distributed completely at random to the treatments subject to the condition that the ith treatment occurs ni times. Randomization assumes that extraneous factors do not continuously influence one treatment.
Randomization and the Randomization Test: Two Sides of the Same Coin
Published in Vance W. Berger, Randomization, Masking, and Allocation Concealment, 2017
This implies that, on average, all treatment averages are identical, and in this sense, the groups in a completely randomized design are equated. In other words, the bias due to potentially confounding variables is, “in expectation,” eliminated.
Insights in nodule-inhabiting plant growth promoting bacteria and their ability to stimulate Vicia faba growth
Published in Egyptian Journal of Basic and Applied Sciences, 2022
Amr M. Mowafy, Mona S. Agha, Samia A. Haroun, Mohamed A. Abbas, Mohamed Elbalkini
The strains were obtained in LB media incubated at 30°C with 150 rpm for 1–2 days to get O.D = 1. The media effect was eliminated by centrifugation at 6000 rpm for 20 min at 4°C and the cells were resuspended in tap water and the O.D was checked to be = 1. In combined treatments, equal volumes of bacterial cultures were mixed after re-suspension in tap water. The whole experiment was conducted in three independent trials. The plants of each group were harvested after 50 days, and the morphological characteristics of each plant were recorded. The number of nodules, dry shoot and root weights, photosynthetic pigments [24], carbohydrates fractions in shoot and root as glucose [25], sucrose [26] and polysaccharides [27] and nitrogen fractions in shoot and root ammonia nitrogen [28], amino nitrogen [29] and total nitrogen [30]. All the experiments were performed in triplicate. The data collected were statistically analyzed using a completely randomized design in the case of the pot trials. The means were compared using the least significant difference test. The correlation coefficients between a pair of the trait were determined and the significance of the correlation determined using ANOVA. All the statistical tests were performed at p < 0.05, using the software COSTAT.
Effect of resistance training combined with β-glucan ingestion on bone of ovariectomized mice
Published in Climacteric, 2022
D. A. Galdino-Alves, G. J. de Sá Pereira, N. de Oliveira Bertolini, R. D. Ferreira, M. de Souza Santos, B. R. Barrioni, M. de Magalhães Pereira, E. F. Andrade, B. Del Bianco-Borges, L. J. Pereira
This study was approved by the Animal Ethics Committee (CEUA/UFLA – protocol 039/18). Eight-week-old female C57BL/6J mice (23 ± 1.6 g) were used. The animals were kept in collective polyethylene cages with four animals in each in a room with standard conditions of temperature (22 ± 2° C), humidity (45 ± 15%) and light/dark cycles (12/12 h). The mice received commercial feed and water ad libitum throughout the experiment. The sample size was calculated to achieve the minimum number of animals needed to attain statistical significance of p < 0.05 with an 80% probability. Considering a large effect size (partial η2 > 0.14) and test power above 80% for the treatment effect, a minimum of five animals per group was required. The experiment followed a completely randomized design. Mice were randomly distributed into six groups with eight animals each: sham-operated group (SHAM); OVX + saline (SAL) group; OVX + alendronate (ALE) group; OVX + BG group; OVX + RT group; and OVX + BG + RT group. Animals of the ALE group received doses of 0.1 mg/kg ALE by gavage [12] as conventional therapy (positive control).
Effect of zeolite on absorption and distribution of heavy metal concentrations in roots and shoots of wheat under soil contaminated with weapons
Published in Toxin Reviews, 2021
Ali Azogh, Seyed Keyvan Marashi, Teimour Babaeinejad
For investigation of absorption and distribution of heavy metals in shoot and root of wheat under soil contaminated with war weapons, a pot experiment was conducted at the Agricultural Research Station, Ahvaz University, Iran during 2015–2016 crop seasons. The field is located at latitude 31° and 19 min and longitude of 48° and 41 min east with a height of 22 m above sea level. The climate is considered to be arid and semi-arid. The average annual rainfall is 250 mm and the average temperature is 23° based on the 20-years weather data. This experiment was conducted as a factorial based on completely randomized design with three replications. The treatments consisted of non-application of zeolite (zero) (control) (a1), 0.5 (a2) and 1.5 (a3), and 2.5 (a4) percent of the soil weight and two levels of soil including non-contaminated soil (b1) and soil contaminated with war weapons (b2).