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Distribution, Biology, and Bio-Diversity of Fenugreek
Published in Dilip Ghosh, Prasad Thakurdesai, Fenugreek, 2022
A study about the variation in chromosome number in fenugreek was also carried out by Joshi and Raghuvanshi (1968) and reported an extra B chromosome in some genotypes of the fenugreek. The presence of the extra B chromosome in the genotypes affected growth and development with varying behaviors (Petropoulos, 2002). The chromosomes are large and varying sizes (longest = 26.28 μm and shortest = 13.52 μm). Reasat et al. (2002) offer a good scope for cytogenetic and molecular studies.
Chromosome Pairing and Fertility in Plant Hybrids
Published in Christopher B. Gillies, Fertility and Chromosome Pairing: Recent Studies in Plants and Animals, 2020
Further studies showed that the effect of B chromosomes could, in fact, be modified by the background genotype.69,72,74 In other words, genotypes of L. perenne and L. rigidum were discovered which could impose their own constraints upon homoeologous association, even in the absence of B chromosomes.70 In the amphidiploid, the genes promote homologous bivalent formation and act in a way similar to the Ph locus of wheat. The identification of a similar diploidizing genotype of L. multiflorum66 is seen as the only means of generating stable agronomically desirable amphidiploids with L. perenne,64 in view of the ineffectiveness and non-Mendelian inheritance of B chromosomes in this material.
Repeated DNA Sequences and Polyploidy in Cereal Crops
Published in S. K. Dutta, DNA Systematics, 2019
During evolution and in the process of speciation, changes in the amount of DNA per cell may arise due to: Polyploidy, which involves duplication of the entire basic chromosome complement of the species or of hybrids between two species as in amphiploidy.Aneuploidy, i.e., addition or deletion of some chromosomes.Changes in the number of accessory or so-called B chromosomes.Gains or loss of DNA without any change in the number of chromosomes.72
G6PD A- is the major cause of G6PD deficiency among the Siddis of Karnataka, India
Published in Annals of Human Biology, 2020
Rati Devendra, Vinod Gupta, Somashekhar S. Biradar, Pradeep Bhat, Shantharam Hegde, S. L. Hoti, Malay B. Mukherjee, Harsha V. Hegde
In order to further explore the ancestry of Sub-Saharan Africa in the Siddi chromosome, the (AC)n and (CTT)n microsatellites were analysed in 45 G6PD A- individuals, along with 8 G6PD B Siddi individuals who agreed to participate in the study. The (AC)n microsatellite repeat sequence for the G6PD B chromosomes (n = 8) was found to be (TA)5(AA)1(TA)6(CA)6(CT)1(CA)1(TA)1(CA)10, corresponding to 178 bp repeat. Siddis with the G6PD A- chromosomes (n = 45) were associated with (TA)5(AA)1(TA)9(CA)10 corresponding to the 166 bp repeat, which is similar to the Sub-Saharan African G6PD A- variant. The (CTT)n repeat sequence in the G6PD B chromosomes was found to be (CTT)11 (ATT)7, corresponding to the 198 bp repeat, while all the Siddis with G6PD A- chromosomes were associated with (CTT)11(ATT)6, corresponding to the 195 bp allele, which is very similar to the African G6PD A- chromosome.
Ameliorative effects of curcumin towards cyclosporine-induced genotoxic potential: an in vitro and in silico study
Published in Drug and Chemical Toxicology, 2018
Ankita J. Shah, Sivakumar Prasanth Kumar, Mandava V. Rao, Himanshu A. Pandya
A total of 100 consecutive metaphase plates per culture were analyzed to score SCEs and differentially stained metaphase plates (first division – M1, second division – M2 and third or successive divisions – M3). Metaphases in their second in vitro division were selected for scoring of SCEs on the basis of the spreading of chromosomes and differentiation of chromatids. In these preparations, cells dividing for the first (M1), second (M2), third or successive (M3) divisions in cultures containing BrdU were designated by the differential staining pattern of sister chromatids. The M1 cells contained chromosomes with both sister chromatids stained uniformly dark. The M2 cells contained only differentially stained chromatids with one chromatid darkly stained and its sister chromatid lightly stained, whereas M3 cells constituted three distinct chromosomal/chromatid features: (a) both sister chromatids stained lightly, (b) chromosomes with a dark and a faint chromatid or (c) chromosomes with a portion that has sister chromatid differentiation and the remaining portion that has both lightly stained chromatids.