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Metagenomic analysis of intestinal mucosa revealed a specific eukaryotic gut virome signature in early-diagnosed inflammatory bowel disease
Published in Gut Microbes, 2019
Federica Ungaro, Luca Massimino, Federica Furfaro, Valeria Rimoldi, Laurent Peyrin-Biroulet, Silvia D’Alessio, Silvio Danese
Being the relative composition of viral communities more important than the absolute number of viral reads18,23, we then analyzed the fraction of specific viral reads on the total viral reads (hereafter referred as relative sequence abundances), rather than the absolute Reads Per Kilobase of transcript, per Million mapped reads (RPKM). By excluding prokaryotic-infecting viruses and viral entities with relative abundances lower than 1%, we found the Herpesvirales order to be the main component of both IBD- and Ctrl-gut viromes (~ 40% of total orders detected), followed by Picornavirales and Tymovirales (~ 40% of total orders detected). However, no statistically significant variation was observed (Figure 1D).