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Perinatal infections
Published in Louise C Kenny, Jenny E Myers, Obstetrics, 2017
The diagnosis of primary infection with toxoplasmosis during pregnancy is made by the Sabin Feldman dye test. Enzyme-linked immunosorbant assays (ELISAs) are available for IgM antibody. However, IgM may persist for months or even years, so often serial testing for rising titres is necessary. If suspicion of congenital toxoplasmosis has arisen because of an abnormal ultrasound scan of the fetus, an amniocentesis can be performed. Polymerase chain reaction (PCR) analysis of amniotic fluid is highly accurate for the identification of T. gondii.
The relation between serum Toxoplasma gondii IgG antibody in children and ADHD and its severity
Published in Psychiatry and Clinical Psychopharmacology, 2019
İsmail Akaltun, Tayfun Kara, Hamza Ayaydın, Behiye Alyanak, Hayati Beka, Ali Ağaçfidan
Blood specimens were collected from the children included in the study, and anti-Toxoplasma IgG antibody levels were investigated using the Enzyme-Linked Immunosorbent Assay (ELISA) method. Five-milliliter specimens collected under sterile conditions were centrifuged at 1000 rpm for serum separation and were stored at -30° C until the time of assay. Serum Toxoplasma antibodies were measured in a 1:20 dilution based on National Committee on Clinical Laboratory Standards, and for ELISA tests, absorbance values >1.1 were regarded as positive. Absorbance values <0.9 were regarded as negative, while values between 0.9 and 1.1 were considered threshold. Repeat tests were performed after 2–3 weeks for cases assessed as threshold. Latent Toxoplasma infection (positive IgG, negative IgM) established by ELISA were confirmed using the Sabin–Feldman dye test (Abbott Laboratories Wiesbaden, Germany). A positivity index >16 was adopted for the Sabin–Feldman dye test [29].
The relationship between Toxoplasma gondii IgG antibodies and generalized anxiety disorder and obsessive-compulsive disorder in children and adolescents: a new approach
Published in Nordic Journal of Psychiatry, 2018
İsmail Akaltun, Soner Sertan Kara, Tayfun Kara
Blood specimens were collected from the children enrolled in the study, and anti-toxoplasma IgG antibody levels were measured using the enzyme-linked immunosorbent assay (ELISA) method. Five-milliliter blood specimens collected under sterile conditions were centrifuged at 1000 rpm for serum separation and then stored at −30 °C until analysis. For the ELISA tests, absorbance values >1.1 at measurement of serum toxoplasma antibodies at a dilution of 1:20 based on National Committee on Clinical Laboratory Standards were regarded as positive. Absorbance values <0.9 were regarded as negative and values between 0.9 and 1.1 were regarded as threshold. Another test was employed after 2–3 weeks for cases regarded as threshold. Latent toxoplasma infection (positive IgG, negative IgM) shown by ELISA was confirmed using the Sabin–Feldman dye test (Abbott Laboratories, Wiesbaden, Germany). Values >16 were adopted as a positivity index for the Sabin–Feldman dye test [33].