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Order Blubervirales: Core Protein
Published in Paul Pumpens, Peter Pushko, Philippe Le Mercier, Virus-Like Particles, 2022
Paul Pumpens, Peter Pushko, Philippe Le Mercier
The core gene of duck hepatitis B virus (DHBV), a representative of the Avihepadnavirus genus, was expressed in E. coli (Schlicht and Schaller 1989; Zheng J et al. 1992) and human hepatoma HepG2 cell line (Schlicht and Schaller 1989). It should be noted that the Orthohepadnavirus but not Avihepadnavirus cores are practically identical and demonstrate 85–87% similarity at the aa level. Moreover, the HBV, WHV, and GSHV, but not DHBV core proteins are able to crossoligomerize into mixed particles, when expressed in Xenopus oocytes (Chang et al. 1994).
Ganciclovir and Valganciclovir
Published in M. Lindsay Grayson, Sara E. Cosgrove, Suzanne M. Crowe, M. Lindsay Grayson, William Hope, James S. McCarthy, John Mills, Johan W. Mouton, David L. Paterson, Kucers’ The Use of Antibiotics, 2017
Camille E. Beauduy, Mark A. Jacobson
Duck hepatitis B virus replication in primary hepatocytes was inhibited by GCV during continuous short-term treatment of the cultures, but GCV was less efficient in inhibiting replication during longer-term exposure (Shaw et al., 1994). GCV treatment of ducks congenitally infected with duck hepatitis B qualitatively decreased serum virus DNA levels, although circulating duck hepatitis B virus surface antigen levels did not decline (Luscombe et al., 1994). Markers of hepatitis B virus infection, including hepatitis B virus DNA and viral DNA polymerase, have been reported to fall to undetectable levels in patients during therapy with GCV (Locarnini et al., 1989).
Yeast-inspired drug delivery: biotechnology meets bioengineering and synthetic biology
Published in Expert Opinion on Drug Delivery, 2019
Chinnu Sabu, Panakkal Mufeedha, Kannissery Pramod
The linking of antigen takes place by partial oxidation of yeast shell by sodium periodate (NaIO4). The partial oxidation results in the presence of an aldehyde group which will react with the primary amine group in protein antigen. In the case of cancer immunotherapy, dendritic cells are major components responsible for immunity induction and for control of tolerance. β-glucan particles are taken up by dendritic cell by the process of phagocytosis and internalization of associated proteins [62]. Recently, chimeric virus-like particle (CVLP) is produced using membrane integral small surface protein of the duck hepatitis B virus as CVLP scaffold and yeast as the heterologous expression host. The CVLP platform is efficient for antigen expression and is a promising candidate for the development of future vaccines [65].
A comprehensive review on phytochemistry, pharmacology, and flavonoid biosynthesis of Scutellaria baicalensis
Published in Pharmaceutical Biology, 2018
Zi-Long Wang, Shuang Wang, Yi Kuang, Zhi-Min Hu, Xue Qiao, Min Ye
Wogonin could suppress HBV antigen secretion with an IC50 of 4 μg/mL for both HBsAg and HBeAg in the human HBV-transfected liver cell line HepG2.2.15, and was more potent than lamivudine. In vivo, wogonin (i.v. for 10 d) could reduce plasma duck hepatitis B virus (DHBV) DNA level in the liver of DHBV-infected ducks with an ED50 of 5 mg/kg, via inhibition of DHBV DNA polymerase and thus reducing the relaxed circular and linear forms of DHBV DNA (Guo et al. 2007).