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Can rose apple leaf be developed for antileucorrhoea and antidandruff?
Published in Ade Gafar Abdullah, Isma Widiaty, Cep Ubad Abdullah, Medical Technology and Environmental Health, 2020
S. Suwendar, F. Lestari, S.P. Fitrianingsih, D. Mardliyani, N. Fitriani
Simplicia was made by drying without direct sunlight (Purnomo & Indarti 2017). Extract was made by maceration (Potluri et al. 2013). Antifungal activity tests were carried out on Candida albicans and Pityrosporum ovale in vitro. All stages were carried out with aseptic techniques. The activity test was carried out using the agar diffusion method in order to use well techniques using a series of test concentrations (in % w/v) Antifungal activity was stated by MIC. Determination of MIC was carried out by the agar dilution method. The concentration of the test on the determination of the MIC was based on the results of the activity test (Sharma et al. 2011, Kandimalla et al. 2016). Fluconazol was used as a comparison. Dimethylsulfoxide (DMSO) was used as a control.
Clindamycin and Lincomycin
Published in M. Lindsay Grayson, Sara E. Cosgrove, Suzanne M. Crowe, M. Lindsay Grayson, William Hope, James S. McCarthy, John Mills, Johan W. Mouton, David L. Paterson, Kucers’ The Use of Antibiotics, 2017
In a multicenter U.S. survey, for the years 1997–2007, 6574 isolates from 13 medical centers were analyzed for resistance to frequently used anti-anaerobic antimicrobials (Snydman et al., 2010). The MICs of the antimicrobials studied were determined via agar dilution in accordance with CLSI recommendations. For the most recent 3 years of the survey (2005–2007), resistance to non–B. fragilis Bacteroides species for clindamycin was reported at > 40%. Specifically, clindamycin resistance for B. fragilis was 23.9%, for B. thetaiotaomicron was 39.8%, for B. vulgatus was 42.6%, for B. ovatus was 45.4%, and for Bacteroides nordii was 25.9%.
Antifungal Drugs and Susceptibility Testing of Fungi
Published in Rossana de Aguiar Cordeiro, Pocket Guide to Mycological Diagnosis, 2019
Débora de Souza Colares Maia Castelo-Branco, Glaucia Morgana de Melo Guedes, Marcos Fábio Gadelha Rocha
The real quantitative methods are those that test serial drug dilutions in order to find the concentration that inhibits fungal growth. These methods are generically known as agar or broth dilution assays. Agar dilution assays can be carried out in Petri dishes containing nutrient agar and increasing drug concentrations. Broth dilution assays, on the other hand, are performed in glass slants, using large volumes of growth medium and drug, or in microtiter plates (broth microdilution), miniaturizing the procedures, making them cheaper and more practical (Figure 2.1).
Preparation and optimization of aloe ferox gel loaded with Finasteride-Oregano oil nanocubosomes for treatment of alopecia
Published in Drug Delivery, 2022
Khaled M. Hosny, Waleed Y. Rizg, Eman Alfayez, Samar S. Elgebaly, Abdulmohsin J. Alamoudi, Raed I. Felimban, Hossam H. Tayeb, Rayan Y. Mushtaq, Awaji Y. Safhi, Majed Alharbi, Alshaimaa M. Almehmady
In the beginning, an agar dilution method was utilized in order to determine the minimal inhibitory concentration (MIC) values (Lamlertthon et al., 2007). To obtain the appropriate concentration range of tested material (0.01–3.0% V/V), different dispersions of FI-Or-NCu were introduced aseptically to 10 mL aliquots of sterile molten agar containing 0.5% tween 80. Then, the obtained solutions were vortexed for 30 seconds or until completely dispersed. Then, the solution was immediately poured into sterile Petri dishes then allowed to be placed for 60 min. The organisms that will be tested P. acne (1 L) from the prepared inoculum was added to the plates, which were allowed until the inoculum had set before being incubated at 37 °C for 72 h under anaerobic conditions as stated before. Subsequently, the utilized plates were observed and recorded for the presence or absence of growth. The results showed that the MIC was noted as the lowest concentration of test materials where the absence of growth was observed.
Activity of five antimicrobial peptides against periodontal as well as non-periodontal pathogenic strains
Published in Journal of Oral Microbiology, 2020
Katharina Enigk, Holger Jentsch, Arne C. Rodloff, Klaus Eschrich, Catalina-Suzana Stingu
The antimicrobial activity of the peptides was determined by an agar dilution method according to EUCAST which represents the gold standard for susceptibility testing of anaerobic bacteria [23]. Although the agar dilution method is labor intensive and time-consuming, the advantage in comparison to the broth dilution method is the possibility to test a high number of strains in one experiment.