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Lymphocytes and Pulsing Magnetic Fields
Published in Andrew A. Marino, Modern Bioelectricity, 2020
Ruggero Cadossi, Giovanni Emilia, Giovanni Ceccherelli, Guiseppe Torelli
The lymphocytes are classified as activated lymphocytes (which include blast and stimulated lymphocytes) and non-stimula ted lymphocytes (Figure 1). The mitotic index (MI) (number of observed mitosis divided by the number of counted cells) is also determined.
Antimitotic and toxicogenetic action of Stevia urticifolia aerial parts on proliferating vegetal and mammalian cells: in vitro and in vivo traditional and replacement methods
Published in Journal of Toxicology and Environmental Health, Part A, 2022
Paulo Michel Pinheiro Ferreira, Ian Jhemes Oliveira Sousa, Kamilla Nunes Machado, Leonel Antônio da Silva Neto, Milena Monteiro de Freitas, Ingredy Lopes dos Santos, Débora Caroline do Nascimento Rodrigues, Rayran Walter Ramos de Sousa, Antonielly Campinho dos Reis, Maria Luisa Lima Barreto do Nascimento, Ag-Anne Pereira Melo de Menezes, Andréa Mendes do Nascimento, José Roberto de Oliveira Ferreira, Ana Paula Peron, João Marcelo de Castro e Sousa
Root growth was significantly inhibited by EtAcSur (33.2 ± 8.1, 30.2 ± 8.1 and 64.3 ± 5.4%) at concentrations of 25, 50 or 100 µg/ml (1.53 ± 0.1, 1.54 ± 0.1 and 1.07 ± 0.1 cm, respectively) compared to negative control (1.99 ± 0.1 cm). Copper sulfate-treated bulbs exhibited roots with 0.36 ± 0.02 cm and growth inhibition of 86.2 ± 1.3% (Figure 3). This inhibition of root growth was corroborated by MI regression at 25, 50 and 100 µg/ml (33 ± 4.4, 28.3 ± 3.4 and 21.1 ± 1.6%, respectively) in comparison with negative control (68.7 ± 3.4%), and modifications in cycle phases, including increased number of cells in interphase and reduced amount of mitosis (prophase, metaphase, anaphase and telophase) in a similar way found for positive control (Table 1). Mitotic index is an important indicator of cell proliferation (Fernandes, Mazzeo, and Marin-Morales 2007; Leme and Marin-Morales 2009). The observed decrease was linked to inhibition of DNA synthesis or blockade of cell cycle, which delays mitosis progression or alternatively induces cell to G0 as noted with EtAcSur, preventing returning to the cell division.
Coarse-grained modeling of cell division in 3D: influence of density, medium viscosity, and inter-membrane friction on cell growth and nearest neighbor distribution
Published in Soft Materials, 2020
Pranav Madhikar, Jan Åström, Jan Westerholm, Björn Baumeier, Mikko Karttunen
Mitotic index () provides a simple measure to characterize cell proliferation.[76–78] It is often used to measure cancer cell proliferation and its response to cancer treatment.[77,79–81] The mitotic index is related to the cell growth rate[76,82] and defined as the fraction of cells that are in some phase of mitosis at a certain moment in time. The fraction is calculated at time intervals approximately the same as the life cycle of the cells being studied. Since CellSim3D does not simulate mitosis completely, the mitotic index is simply defined as , where is the number of new cells created in the time interval (here, 1000 time steps were used), and is the total number of cells at time . In a system with no cell deaths and ideal growth conditions (i.e., no competition for any growth factors and/or nutrients), it is assumed that the mitotic index and the growth rate are equivalent, that is,