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Recombinant vaccines: Gag-based VLPs
Published in Amine Kamen, Laura Cervera, Bioprocessing of Viral Vaccines, 2023
Laura Cervera, Irene González-Domínguez, Jesús Lavado-García, Francesc Gòdia
Several compounds were described to enhance Gag-based VLPs. [105]. Two main groups of transfection enhancers were tested. One group was selected on the basis that they can either facilitate the entry of PEI/DNA transfection complexes into the cell or cell nucleus. Another group was selected according to their capacity to increase the levels of gene expression. Among the eight reagents tested (trichostatin A, valproic acid, sodium butyrate, DMSO, lithium acetate, caffeine, hydroxiurea, and nocodazole), an optimal combination of compounds exhibiting the greatest effect on gene expression was identified. The addition of 20 mM lithium acetate, 3.36 mM of valproic acid, and 5.04 mM of caffeine increased production levels by fourfold, while maintaining cell culture viability at 94%.
Nanotechnology Applications in Nanomedicine: Prospects and Challenges
Published in Khalid Rehman Hakeem, Majid Kamli, Jamal S. M. Sabir, Hesham F. Alharby, Diverse Applications of Nanotechnology in the Biological Sciences, 2022
Arpita Dey, Smhrutisikha Biswal, Somaiah Sundarapandian
Metal nanostructures: Metal nanostructures, especially transition metal-based NPs such as gold nanoparticles (AuNPs), serve as carriers of therapeutic cargos, having diagnostic and medicinal properties of their own. For example, the plasmon behavior of AuNPs is applied in photothermal therapy and imaging techniques as scaffolds for cell surface sensing. Passive tumor targeting and active targeting of gold-based-nanomedicine have reached anticancer clinical trials. Nanocrystalline silver is widely established as antimicrobial agents. Histone deacetylases (HDACs) inhibitors like SAHA and sodium butyrate incorporated with gold nanoparticles showed good therapeutic potential in cancer cells.
mcl-PHA
Published in Martin Koller, The Handbook of Polyhydroxyalkanoates, 2020
Camila Utsunomia, Nils Hanik, Manfred Zinn
Employing P. putida KTHH06 in combination with a sequential feeding strategy, Hu et al. [110] produced copolymers of 3HB and 4HB from sodium butyrate and γ-butyrolactone, respectively. The obtained D value for the dyad analysis was found to be around 356, and, together with 2D NMR 1H-13C HMBC experiments showing covalent bond formation between 3HB and 4HB monomers, the results were taken as solid evidence of the existence of the PHB-b-P4HB block copolymer. Further thermal analysis by DSC and mechanical testing (tensile testing) allowed them to differentiate between the produced polymers and random copolymer as well as blends of homopolymers.
In vitro chemo-protective effect of Eisenia foetida coelomic fluid against histone deacetylase inhibitor-induced oxidative toxicity in breast cancer cells
Published in International Journal of Environmental Health Research, 2022
Asuman Deveci Özkan, Janiah Alimudin, Yasemin Kilciler, Burcu Yuksel, Ozlem Aksoy, Zeynep Betts
Sodium butyrate (NaBu), which is used as a chemotherapeutic agent in the treatment of breast cancer, which is the most common cancer in women in the world, is a short-chain fatty acid found in humans, produced from dietary fibre (Yuksel et al. 2022). It inhibits proliferation in breast cancer cell lines and induces morphological changes, causing growth arrest, differentiation and apoptosis in cells. (Huang et al. 2017). In cancer treatment, necrotic cell death may disrupt membrane integrity and cause the release of mediators associated with inflammation and immunosuppression into the extracellular environment. This situation may pose a threat to the patient by promoting carcinogenesis. Therefore, it is very important to eliminate or reduce the high cytotoxicity of NaBu for both neoplastic and normal cells. Our study investigated the chemoprotective effect of coelomic fluid obtained from Eisenia foetida on the toxicity of NaBu, a histone deacetylase inhibitor, in breast cancer. For this purpose, the cytotoxic and chemoprotective effects of ECF on breast cancer cell viability were determined. To evaluate the effect of ECF on the oxidative toxicity induced by NaBu in MCF-7 cells, changes in ROS production levels and expression of cell death and ROS-related genes (Bax, Bcl-2, SOD and CAT) were detected.