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Conjugated Poly/Oligo-Electrolytes for Cancer Diagnosis and Therapy
Published in John R. Reynolds, Barry C. Thompson, Terje A. Skotheim, Conjugated Polymers, 2019
Lingyun Zhou, Guillermo C. Bazan, Shu Wang
As we have mentioned above, CCP-based SNP detection reduces cost and complexity, while the combination of the molecular wire effect and FRET is capable of enhancing the detection signal. Utilizing PFP1 with a similar procedure, more than one site of mutation can be detected at the same time. Furthermore, the results can be visualized by human-unaided eyes. Mutations on PIK3CA gene have been discovered in different human cancers. Specifically, E545K (G1633A) and H1047R (A3140G), two of four hotspot mutations (E542K (G1624A), E545K (G1633A) in exon 9 (helical domain), and H1047R (A3140G), H1047L (A3140T) in exon 20 (kinase domain)), were chosen as detection targets. Two SBE on E545K primer and H1047R primer were conducted utilizing dATP-TR (TexRed label), dUTP-Fl (fluorescein label) and Taq polymerase. The 3′-terminal base of two primers individually complimented the mutation site of E545K and H1047R, but not the wild type. After adding PFP1, different FRET phenomena (PFP1→Fl, PFP1→TR, Fl→TR) occur depending on single E545K mutation, single H1047R mutation, both E545K and H1047 mutation, and wild type. The fluorescent color is also varied from each situation and can be distinguished by observation (Figure 24.10).86
Investigate the role of PIK3CA gene expression in colorectal polyp development
Published in Egyptian Journal of Basic and Applied Sciences, 2023
Ameer Ali Imarah, Rana Ahmed Najm, Haider Ali Alnaji, Saleem Khteer Al-Hadraawy, Abbas F. Almulla, Hussein Raof Al-Gazali
The gene PIK3CA, encoding the alpha catalytic subunit of phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K), plays an important role in developing many malignancies. The PIK3CA gene is found at locus 3q26.32 on the long arm of chromosome number 3. PIK3CA mutations have been found in many malignancies, including the most common cancers, such as breast, endometrial, and colorectal cancers [23]. The PIK staining in the current study shows that all control group cases were negative, while in the patient’s group, all cases were positive, as shown in Figures (8–11). These results may indicate a possible role of the PIK3CA gene in tumor development, and these results are consistent with a study achieved by [24].