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Magnetic Nanoparticle-Based Biosensing
Published in Nguyễn T. K. Thanh, Clinical Applications of Magnetic Nanoparticles, 2018
Kai Wu, Diqing Su, Yinglong Feng, Jian-Ping Wang
In summary, this work extends the application of GMR-based assay for virus detection. As nucleoprotein is localized within the virus particle, a non-ionic, nondenaturing detergent IGEPAL CA-630 is used to disrupt the virus particles in the sample. The results demonstrated that the GMR biosensor is able to detect viral concentration with dynamic range of 3 orders of magnitude (1.5 × 102 to 1.0 × 105 TCID50/mL). This is relevant to nasal samples of infected swine, which has been reported to contain 103 to 105 TCID50/mL viral particles.120
Exposure to lead on expression levels of brain immunoglobulins, inflammatory cytokines, and brain-derived neurotropic factor in fetal and postnatal mice with autism-like characteristics
Published in Journal of Toxicology and Environmental Health, Part A, 2021
KyeongMin Shin, GyeongDong Lim, Young-Seoub Hong, SoNam Kim, SoRyeon Hwang, JaeHee Lee, SoJung Sin, AhRang Cho, YeonGyeong Kim, Ravi Gautam, JiHun Jo, Manju Acharya, Anju Maharjan, DaEun Lee, Pramod B. K C, ChangYul Kim, Yong Heo, Hyoung-Ah Kim
Fetal and postnatal brain homogenates were prepared as described previously (Hwang et al. 2015; Kim et al. 2016a; Liu et al. 2020). Briefly, for fetal brain homogenates, the meningeal layers were peeled off to remove the vasculature, and subsequently brain tissues were homogenized in buffer (pH 8) containing 20 mM Tris, 100 mM NaCl, 2 mM Na2EDTA, 1% Igepal CA-630 nonionic detergent in which protease inhibitor cocktail (Sigma) was added as 1% concentration, followed by sonication for 10 min. The supernatants were collected following 30 min centrifugation (16,000 × g) at 4°C and stored at −80°C prior to use. For postnatal brain homogenates, whole brains were collected following intracardiac perfusion with warm phosphate buffered saline (PBS; 50 ml/mouse pH 7–7.6, Lonza, Walkersville, MD, USA). Cardiac blood was collected prior to perfusion under inhalational anesthesia with isoflurane (Hana Pharm, Seoul, Korea). Brain tissues were homogenized for 30 min at 16,000 g at 4°C and protein extracts prepared as described for fetal lysates. Protein concentrations in the homogenates were determined using a BCA protein assay kit (Thermo, Rockford, IL, USA; Kobos et al. 2020).