Hematoxylin

Hematoxylin

Disease-Inspired Feature Design for Computer-Aided Diagnosis of Breast Cancer Digital Pathology Images

Published in de Azevedo-Marques Paulo Mazzoncini, Mencattini Arianna, Salmeri Marcello, Rangayyan Rangaraj M., Medical Image Analysis and Informatics: Computer-Aided Diagnosis and Therapy, 2018

Jesse Knight, April Khademi

More commonly used staining protocols, in all types of cancers and tissue analysis, include those that employ the H&E stains. Instead of considering specific proteins or biomarkers, H&E stains make visible the structural characteristics of cells and tissue [13]. Hematoxylin is a bluish (or purple) stain that has an affinity to acidic structures like nucleic acids (nuclei). Eosin usually has an affinity to th e background stroma and is generally colored pink.

The effects of a high-fat diet on the liver of pregnant albino rats and their developing offspring

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Journal Information

Published in Egyptian Journal of Basic and Applied Sciences, 2023

Abdelalim A. Gadallah, Abdullah R. Almasari

Formalin-fixed liver tissue sections, 5 μm in thickness, were placed on glass slides coated with polylysine and kept at room temperature. The tissue sections were subsequently exposed to the PCNA antibody (Thermo Fisher Scientific, Fremont, CA, USA, Cat. No. 31–033) and the caspase-3 antibody obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, NB100–56116). In order to enhance background staining, the tissue sections were treated with hematoxylin as a counterstain. Subsequently, the specimens were examined and photographed. The generated images were processed and analyzed using Video Test morphology software. The software facilitated the measurement and recording of the percentage area for the analyzed images.

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The effects of a high-fat diet on the liver of pregnant albino rats and their developing offspring