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Cyanobacterial Phycobiliproteins – Biochemical Strategies to Improve the Production and its Bio Application
Published in Sanjeet Mehariya, Shashi Kant Bhatia, Obulisamy Parthiba Karthikeyan, Algal Biorefineries and the Circular Bioeconomy, 2022
Khushbu Bhayani, Imran Pancha, Sandhya Mishra
In a view of marvellous biotechnological application and commercial optimization, pigment's purity makes a major contribution; hence, a preferable purification method is needed. After extraction, PBP concentration is moderately low, ergo the purification step must ameliorate the purity of PBPs. The purification protocol is made up of different steps. Routine methods contain a combination of ammonium sulphate precipitation and chromatography technique (Table 11.4). Ammonium sulphate precipitation involves two steps; in the first step, it precipitates impurities up to 25–30% (W/V) ammonium sulphate, and in the second step, it precipitates impurities up to 60–70% (w/v). It removes cell debris and other impurities, so this step is necessary before chromatographic purification of PBPs. For further purification of PBPs, researchers have developed different column chromatography techniques, e.g., ion exchange chromatography, gel filtration chromatography, expanded bed adsorption chromatography, hydrophobic interaction chromatography, and hydroxyapatite chromatography (Abalde et al., 1998; Soni et al., 2006; Patil et al., 2006; Johnson et al., 2014; Lauceri et al., 2018). Gel filtration chromatography (size-exclusion chromatography) separates proteins according to their size, and ion exchange chromatography separates proteins according to their affinity toward ions. These two are common methods people are applying in lab scale. Sonani et al. (2014) have separated CPC, APC, and CPE with greater purity ratio by using a combination of ammonium sulphate precipitation, ion exchange, and size exclusion chromatography. Gradients of ionic strength help to purify PBPs while using ion exchange chromatography, but PBPs elution with different pH gradients is a more fruitful method (Su et al., 2010; Yan et al., 2011; Kumar et al., 2014). In expanded bed adsorption chromatography, protein separates directly from crude extract; thus, it eliminates a few steps from the purification protocol, which is a work-saving protocol (Niu et al., 2010). Aqueous two-phase extraction is globally used for purification of colourful PBPs. It can be applied for large-scale setups, but compared to this method, chromatographic methods are more proficient.
Purification of chitosanases produced by Bacillus toyonensis CCT 7899 and functional oligosaccharides production
Published in Preparative Biochemistry & Biotechnology, 2022
Julia Maria de Medeiros Dantas, Nathália Kelly de Araújo, Nayara Sousa da Silva, Manoela Torres-Rêgo, Allanny Alves Furtado, Cristiane Fernandes de Assis, Renata Mendonça Araújo, José António Teixeira, Leandro de Santis Ferreira, Matheus de Freitas Fernandes-Pedrosa, Everaldo Silvino dos Santos
Our research group has used expanded bed adsorption chromatography (EBA) strategy to purify chitosanases.[13–15] In this context, the present study had as main objectives the increase of the fold purification of chitosanases and the produce bioactive COS with them. A purification protocol using an automatic system was used,[16–18] represents an evolution from the manual system used in past studies[13–15] to purify chitosanases produced by B. toyonensis. After characterization by gel permeation chromatography (GPC) and mass spectroscopy (MS), the COS had its antiedematogenic activity preliminarily investigated through carrageenan-induced paw edema model. Thus, this study novelty relies on the use of a B. toyonensis strain to produce COS with biological activity and the simplification of the oligomers production process to just one step.
Simultaneous production of propionic acid and vitamin B12 from corn stalk hydrolysates by Propionibacterium freudenreichii in an expanded bed adsorption bioreactor
Published in Preparative Biochemistry & Biotechnology, 2020
Peng Wang, Chen Shen, Luwei Li, Jinfeng Guo, Qinqin Cong, Jialin Lu
To eliminate the inhibition of the fermentation by propionic acid, many attempts on the feasibility of changing propionic acid from harmfulness to usefulness have been conducted, such as various in situ product removal techniques, ISPR techniques.[8–11] The use of the ISPR technique to produce other fermentation products such as organic solvents has also been reported.[12,13] Among these ISPR techniques, a highly efficient expanded bed adsorption bioreactor (EBAB) was developed for recovering two products (propionic acid and VB12) in one single fed-batch fermentation process.[14] In fed-batch-EBA fermentation using pure glucose as substrate, the propionic acid and VB12 concentration reached to 52.5 g L−1 and 43.04 mg L−1 at 160 h, respectively. When using the conventional ISPR process like the immobilized cell bioreactor, the immobilized cells would be used repeatedly, which usually led to make the productivity loss over time.[9] EBAB has the regenerative capacity and high mass transfer capacity for stable long-term productivity.[15] However, the cost for the synthesis of VB12 and propionic acid were high, So there is still a need to find more cost-effective raw material to make the production of propionic acid and VB12 production more efficient.
In situ recovery of taxadiene using solid adsorption in cultivations with Saccharomyces cerevisiae
Published in Preparative Biochemistry & Biotechnology, 2023
Giuseppe R. Galindo-Rodriguez, Jorge H. Santoyo-Garcia, Leonardo Rios-Solis, Simone Dimartino
The integration of an external adsorption column for in situ product recovery has been reported to improve productivity. For example, Wang et al. used a fluidized bed column to remove propionic acid during fermentation, resulting in a 1.4 and 1.2-fold higher productivity compared to a bioreactor with an internal column and a bioreactor with dispersed resin, respectively.[36] Tan et al. compared the performance of an expanded bed adsorption column with a dispersed resin system for the in situ removal of acetate to improve α-interferon-2b production and observed higher productivity in the expanded bed adsorption column compared to a system with dispersed resin in the bioreactor.[35]