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Food Production and Processing
Published in Shintaro Furusaki, John Garside, L.S. Fan, The Expanding World of Chemical Engineering, 2019
Sauerkraut fermentations are carried out by several bacterial cultures; in the early stages of this cabbage fermentation, Enterobacter cloacae and Erwinia herbicola may be found together with Leuconostoc mesenteroides which produces lactic acid. About the second day of the fermentation the population of L. mesenteroides increases to about one billion/ml, and the acidity inhibits the enteric organisms. Lactobacillus plantarum succeeds Leuconostoc mesenteroides and produces more lactic acid. It also consumes the bitter mannitol produced by Leuconostoc mesenteroides. Small numbers of Pediococcus cerevisiae and Lactobacillus brevis may often be found during the middle stage of the fermentation (Erickson and Fung 1988).
Optimization and purification of glucansucrase produced by Leuconostoc mesenteroides DRP2-19 isolated from Chinese Sauerkraut
Published in Preparative Biochemistry and Biotechnology, 2018
Renpeng Du, Fangkun Zhao, Lei Pan, Ye Han, Huazhi Xiao, Zhijiang Zhou
Various molecular forms of active glucansucrases are obtained in the extracellular medium, recently described to be a result of proteolytic processing, but rarely optimization of enzyme production by the native strain.[9] Some studies found that carbon source, metals ions, Tween-80, pH value, temperature have significant effect on glucansucrases production.[9] The enzyme can be stabilized by high-molecular-weight dextran, polyethylene glycol, or nonionic detergents. Glucansucrases are inactivated by EDTA and can be reactivated with Ca2+. Glucansucrases are strongly inhibited by metal ions such as Cu2+, Fe3+, and Mn2+.[10] Exploration of natural biodiversity of glucansucrase-producing LAB strains could offer EPS synthesis from sucrose, as a promising source for health, food, and nutritional applications. The aim of the present work was to isolate, screen glucansucrase-producing LAB from Chinese sauerkraut juice, optimize the fermentation conditions and purify glucansucrase, which should be used as potential commercial production of EPS in further research.