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BioMicrobialdiesel Production using Microbes in General
Published in Ozcan Konur, Biodiesel Fuels Based on Edible and Nonedible Feedstocks, Wastes, and Algae, 2021
K. V. V. Satyannarayana, Randeep Singh, I. Ganesh Moorthy, R. Vinoth Kumar
With increasing industrialization and urbanization, the volume of sludge produced from wastewaters has increased drastically (Kumar et al., 2016). The inherent lipid content of sludge makes it ideal feedstock for oleaginous bacteria (Olkiewicz et al., 2014). The biodiesel obtained from oleaginous bacteria shows sludge is a promising option for biodiesel production. Analysis of 5% blends of this biodiesel shows better fuel properties and that it can be used in existing engines without any modifications (Kumar et al., 2018). The use of wastewater for lipid accumulation using Rhodococcus opacus bacteria is a prominent idea for biodiesel production. The bacterial high cell density and lipid-rich biomass result in high biomass content and ‘chemical oxygen demand’ (COD) removal (Goswami et al., 2017a–b).
Bio-oil production from oleaginous microorganisms using hydrothermal liquefaction: A biorefinery approach
Published in Critical Reviews in Environmental Science and Technology, 2022
Tanushree Paul, Arindam Sinharoy, Divya Baskaran, Kannan Pakshirajan, G. Pugazhenthi, Piet N. L. Lens
Growth of oleaginous bacteria, algae and fungi on wastewater is well reported in the literature. Goswami et al. (2019) reported biodegradation of organics by Rhodococcus opacus grown on different industrial wastewaters: pulp and paper, biomass gasification and dairy with a COD removal efficiency of 46.1%, 68.9% and 56.8%, respectively, and biodiesel production from the residual biomass. Similarly, Paul et al. (2019) reported biodegradation of recalcitrants present in refinery wastewater by Rhodococcus opacus followed by a membrane filtration technique for separation of biomass and hydrothermal liquefaction products of the biomass to produce bio-oil. These studies clearly demonstrate the suitability of oleaginous bacteria to utilize industrial wastewater as substrate for bio-oil production.
Real-time lipid production and dairy wastewater treatment using Rhodococcus opacus in a bioreactor under fed-batch, continuous and continuous cell recycling modes for potential biodiesel application
Published in Biofuels, 2018
Nikhil Gupta, N. Arul Manikandan, Kannan Pakshirajan
The dairy industry is a prominent food industry in all developing nations, including India, and it generates a huge volume of wastewater, which often contains high lactose, suspended solids including fat, nutrients such as phosphate, ammonia or minerals [5]. Oleaginous biomass grown using such wastewater is a potential feedstock for biodiesel production [3]. Although algae and yeast are extensively researched for lipid production, the use of bacteria for lipid production is still in its infancy. Recently, wastewater borne bacteria have shown excellent lipid accumulation capacity [3]. However, real time lipid production by bacteria using wastewater for both biodiesel application and wastewater treatment has not been examined. The present study, therefore, focussed on lipid production by Rhodococcus opacus using dairy wastewater as the carbon source in a bioreactor operated under fed batch, continuous and continuous cell recycle modes.