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Stability of Airborne Microbes and Allergens
Published in Christopher S. Cox, Christopher M. Wathes, Bioaerosols Handbook, 2020
Work during the late 1960s and early 1970s7 demonstrated that viruses without structural lipids, such as mengovirus 37A, polio virus, foot-and-mouth disease (FMD) virus and encephalomyocarditis(EMC) virus are unstable as bioaerosols when wet-disseminated and stored in atmospheres below about 70% RH because of their denatured surface structures. The approach compared infectivity of whole virus with that of isolated infectious RNA8 (Figure 6.3). For EMC virus there was evidence of a concomitant loss of hemagglutination activity and of affinity for hemagglutination inhibiting antibodies while viral RNA activity remained unimpaired.
¨Detection of SARS-CoV-2 in wastewater as an early warning in the metropolitan area of the city of Buenos Aires (BAMA).¨
Published in Ribagua, 2023
Alejandro Barrio, Verónica Borro, Marcelo Cicchino, Adriana Morón, Lorena Coronel, Juan Vuolo, Paula Mayón, Ayelén Moroz, Paula Maisa, Sebastián Alcántara, María Torras, Marcos Cervini, Martín Arzeno, Cristian Godoy, Diego Campillay, Nestor Filliel, Ana Salas, Paula Salvio
Since virus losses can occur at the different stages of the process, to control it, a process control virus such as mengovirus (MGV) (MGV standard, CEERAMTOOLS®) was added at the beginning of the process and, when compared with that obtained from the MGV virus without a matrix (standards), obtain the degree of recovery by measuring the extraction efficiency of the same, described in ISO15216-1, 2017 [31]. Validation was carried out using 20 effluent samples collected over the course of two weeks under the same conditions as the samples to be analyzed. Once in the laboratory, 10 μL of MGV was inoculated to those samples, following the same concentration and extraction processes. The extracts obtained were also diluted 1/10.