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Illuminating the cycle of life
Published in Raquel Seruca, Jasjit S. Suri, João M. Sanches, Fluorescence Imaging and Biological Quantification, 2017
Anabela Ferro, Patrícia Carneiro, Maria Sofia Fernandes, Tânia Mestre, Ivan Sahumbaiev, João M. Sanches, Raquel Seruca
Cell-cycle analysis is also of utmost importance in studies concerning cancer cell invasion. Indeed, to target invading cancer cells one must determine the cell-cycle phase, given that most chemotherapeutic agents target S/G2/M cells [118]. In a recent study, Yano and coworkers demonstrated by real-time FUCCI imaging that cancer cells in G0/G1 can migrate further and faster than those at S/G2/M phase of the cell cycle. Furthermore, the authors observed that chemotherapy had little effect on this subpopulation of highly migratory cancer cells, which can explain why chemotherapy is so inefficient in preventing metastasis [118].
Cytotoxicity activity, in silico molecular docking, protein- and DNA-binding study of a new Ni(II) Schiff base complex
Published in Journal of Coordination Chemistry, 2018
Niladri Biswas, Sumit Khanra, Arnab Sarkar, Shamee Bhattacharjee, Deba Prasad Mandal, Ankur Chaudhuri, Sibani Chakraborty, Chirantan Roy Choudhury
Cell cycle analysis is a method in that employs flow cytometry to distinguish cells in different phases of the cell cycle based on fluorescence intensity of the stained cells at certain wavelength. For the determination of cell cycle phase distribution of nuclear DNA, in vitro AGS, A549 cells (1 × 106 cells) were harvested. After making a single cell suspension, cells were fixed with 3% p-formaldehyde, permeabilized with 0.5% Triton X-100, and nuclear DNA was labeled with propidium iodide (PI, 125 μg/mL) after RNase (40 µg/mL) treatment. Cell cycle phase distribution of nuclear DNA determined on FACS Caliber using Cell Quest Software (Becton-Dickinson Histogram display of DNA content (x-axis, PI fluorescence) versus counts (y-axis) has been displayed.
Cytocompatibility and osteogenic differentiation of stem cells from human exfoliated deciduous teeth with cotton cellulose nanofibers for tissue engineering and regenerative medicine
Published in Journal of Biomaterials Science, Polymer Edition, 2022
Rafaella de S. S. Zanette, Leonara Fayer, Eduarda R. de Oliveira, Camila G. Almeida, Cauê R. Oliveira, Luiz F. C. de Oliveira, Carlos M. C. Maranduba, Érika C. Alvarenga, Humberto M. Brandão, Michele Munk
Flow cytometry was performed for cell viability and cell cycle analysis. First, the cells were seeded in a 6-well plate in triplicate at a density of 2 × 105 cells/well and treated with various cotton CNF concentrations (0, 0.1, 1, 10, 50, and 100 µg mL−1) for 24 and 48 h. After each time, the culture medium was removed, and the cells were washed twice with 1 mL of the phosphate-buffered saline solution (pH 7.2) (Gibco Laboratories, UK) and then treated with 0.25% (w/v) trypsin and 0.5 mM ethylenediaminetetraacetic acid (EDTA) (Sigma-Aldrich, St. Louis, MO, USA) for 3 min to allow the cells to detach from the bottom. Trypsin was deactivated with a DMEM medium supplemented with 10% FBS. The cells were collected by centrifugation at 5000g.
Curcumin reduced gold nanoparticles synergistically induces ROS mediated apoptosis in MCF-7 cancer cells
Published in Inorganic and Nano-Metal Chemistry, 2020
Sindhu Kondath, Rama Rajaram, Rajaram Anantanarayanan
Cell cycle analysis determines the percentage of cells in different phases depending upon their DNA content. Cells in their hypodiploid state accumulate in the sub-G1 fraction and are considered apoptotic. The cell population in sub-G1 fraction increases from 7% in control to 20, 40 and 65% on treatment with 23, 45 and 90 µg/ml of cAuNPs respectively (Figure 5b).