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Acute Toxicity Testing by the Dermal Route
Published in Rhoda G. M. Wang, James B. Knaak, Howard I. Maibach, Health Risk Assessment, 2017
Roy C. Myers, Lin val R. DePass
As noted previously, minimal lethal doses or approximate lethal doses may be quite adequate, if the test is conducted carefully. With these methods, administration of higher doses for the sole purpose of increasing numbers of deaths is not necessary. Instead, increasing the amount of biologic data (histopathology, clinical chemistry, etc.) from nonlethal doses may be more desirable.80,120 If we eliminate death as an endpoint altogether, both animal numbers and animal stress can be reduced. Methods such as the British Toxicology Society fixed-dose procedure (discussed previously) have been developed to accomplish this goal and are gaining in acceptance.
Animal Toxicity Studies
Published in Nicola Loprieno, Alternative Methodologies for the Safety Evaluation of Chemicals in the Cosmetic Industry, 2019
For instance, to determine acute oral toxicity an alternative method (the “Fixed Dose Procedure”) not utilizing death as a specific endpoint was introduced.19 It makes use of fewer animals and results in less pain and distress than the classical determination of acute oral toxicity. The fixed dose method is conducted in two stages: in a preliminary study, the effect of various dose administered orally by gavage to single animals of one sex are investigated sequentially; in the main study, the substance is administered orally by gavage to groups of five male and five female animals at one of the preset dose levels (5, 50, 500, or 2,000 mg/kg);
Antidiabetic and antihyperlipidemic effects of a methanolic extract of Mimosa pudica (Fabaceae) in diabetic rats
Published in Egyptian Journal of Basic and Applied Sciences, 2019
Subramani Parasuraman, Teoh Huey Ching, Chong Hao Leong, Urmila Banik
Healthy, adult female SD rats were used for the experiment. The acute toxicity testing was performed by using the fixed-dose procedure. Overnight fasted rats were orally fed with the MEMP in increasing dose levels of 250, 500, 1000 and 2000 mg/kg body weight (n = 3 per dose), respectively. The animals were observed for their behavioral, neurological and autonomic profiles continuously for 24 h. After a period of 24 h, the animals were observed for 14 days for mortality. [22].