China
Africa
Explore chapters and articles related to this topic
Nonclassical Ion Channels and Ischemia
Published in Tian-Le Xu, Long-Jun Wu, Nonclassical Ion Channels in the Nervous System, 2021
There are 28 mammalian TRP channels separated into 6 sub-families: TRPC (where C denotes canonical), TRPM (melastatin), TRPV (vanilloid), TRPA (ankyrin), TRPP (poly-cystin), and TRPML (mucolipin). They share similar structure, but their functions are diverse and display a different activation mechanism. It has been proved that TRPM, TRPV, TRPC, and TRPA channels are located in glial cells. The mammalian TRPC (canonical) family consists of seven members (TRPC1–7) that are organized into four groups: TRPC1, TRPC2, TRPC3/6/7 and TRPC4/5, because they are with sequence homology and functional similarities [40]. The TRP is a superfamily of non-selective cation channels that are widely expressed in mammalian cells [13,41], and they are activated by a multitude of ligands, physical stimuli (e.g. force and temperature), differing local ion concentrations and secondary messengers released via G-protein coupled receptors [42].
The relevant targets of anti-oxidative stress: a review
Published in Journal of Drug Targeting, 2021
Transient receptor potential（TRP）channels are non-selective cation channels across cell membranes. In 1969, Cosens and Manning discovered for the first time that the Trp channel could generate transient rather than continuous spike potential under continuous light stimulation in drosophila with Trp gene mutation [104], hence the name Trp channel. The TRPC channel is one of the important members of the TRP family. It consists of six transmembrane domains (TMs), and the ion channel pore is located between TM5 and TM6. TRPC channels include seven different subtypes, namely TRPC1-7. Except TRPC2, the other six subtypes are widely expressed in a variety of human tissues and cells including cardiomyocytes. In myocardial tissue, TRPC1, TRPC3, TRPC 4 and TRPC 6 are distributed in the mouse sinoatrial node area and pacemaker cells, and TRPC3 and TRPC6 are also expressed in extranodal tissues [105,106].
Screening of rosmarinic acid from Salvia miltiorrhizae acting on the novel target TRPC1 based on the ‘homology modelling–virtual screening–molecular docking–affinity assay–activity evaluation’ method
Published in Pharmaceutical Biology, 2023
Wei Quan, Yuan Wang, Yu-han Chen, Qing Shao, Yang-ze Gong, Jie-wen Hu, Wei-hai Liu, Zi-jun Wu, Jie Wang, Shan-bo Ma, Xiao-qiang Li
Transient receptor potential canonical channel (TRPC) is a kind of Ca2+-permeable non-selective cation channel. Based on the amino acid sequence, the TRPC family is divided into seven isoforms (TRPC1–7, among which TRPC2 is a human pseudogene) (Wang et al. 2020). TRPC is expressed in several organs, including the heart, brain and kidney. Store-operated calcium entry (SOCE), as one of the major pathways of calcium entry, plays an important role in intracellular calcium homeostasis, affecting important cellular processes (Prakriya and Lewis 2015). TRPC1 is also the main ion channel involved in SOCE (Dyrda et al. 2020), and the downregulation of TRPC1 reduces SOCE by approximately 60% (Ambudkar et al. 2017). Recent studies have shown that TRPC1 and Ca2+-related pathways mediated by it are involved in the regulation of many physiological and pathological processes, including neuronal survival (Sun et al. 2017), glomerular mesangial cell contraction (Du et al. 2007), skeletal muscle development (Antigny et al. 2017) and diabetes mellitus (Krout et al. 2017). Additionally, abnormal expression of TRPC1 may be involved in the progression of many cardiovascular system diseases, including myocardial hypertrophy (Inoue et al. 2006), vascular injury and generation (Martin-Bornez et al. 2020) and atherosclerosis (AS) (Hof et al. 2019). According to our experimental results, under the conditions of HL-1 oxygen-glucose deprivation/reperfusion (OGD/R), Ca2+ in cardiomyocytes was not significantly changed after the inhibition of voltage-dependent calcium channel (VDCC) with nifedipine. In contrast, Ca2+ influx was significantly reduced after the application of non-selective TRP channel blocker SKF-96365 or the Trpc1 gene silencing. The above studies suggest that TRPC1 may be involved as an important target in myocardial injury.