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Medicinal Plants: Future Thrust Areas and Research Directions
Published in Amit Baran Sharangi, K. V. Peter, Medicinal Plants, 2023
In general, a very few medicinal plant species are only sexually sterile, but the majority have some sexual fertility. Based on the breeding objectives, any breeding program involves three important steps, i.e., induction of variability, selection of desired traits and propagation and multiplication of new varieties. For meeting the above goal, either of the conventional breeding methods like introduction, selection, hybridization-intra, and interspecific, polyploidy, and mutation, alone or in combination are effective. Additional tools like tissue culture techniques (micropropagation, selection, embryo culture, anther culture, cell suspension culture, and protoplast fusion) and recombination techniques (marker-assisted selection (MAS) and genetic transformation) are also being utilized. At each step, different techniques can be applied which have an impact at plant/population level, cell/tissue level and DNA level.
Edible Vaccine
Published in Hafiz Ansar Rasul Suleria, Megh R. Goyal, Masood Sadiq Butt, Phytochemicals from Medicinal Plants, 2019
Vivek K. Chaturvedi, Sushil K. Dubey, N. Tabassum, M.P. Singh
The idea of mushroom-based EV technology has shown low cost production results, and is rich in nutrition and effective therapeutic therapy against many diseases. These include high biosynthetic capacity with production of immunomodulatory compounds, and the availability of genetic transformation methods. Not many reports have been distributed on the utilization of edible mushroom as a creation stage for biopharmaceuticals. Among the Pleurotus family, P. eryngii species has been designed to assess its potential for generation of biopharmaceuticals, Interleukin-32.13 Other researcher changed mushroom by an articulation of human development hormone quality (hGH) in P. eryngii.38
Mutants as Tools for the Analytical Dissection of Cell Differentiation in Physcomitrella Patens Gametophytes
Published in R. N. Chopra, Satish C. Bhatla, Bryophyte Development: Physiology and Biochemistry, 2019
Philosophical considerations underlying the isolation of abnormal strains, possessing genes which have been mutated by conventional means30 or by insertional inactivation with foreign DNA introduced by a genetic transformation procedure,6,27,32 and their utilization for studying developmental processes are discussed in the first chapter of this volume and elsewhere.6,7,10,21
Echinacea biotechnology: advances, commercialization and future considerations
Published in Pharmaceutical Biology, 2018
Jessica L. Parsons, Stewart I. Cameron, Cory S. Harris, Myron L. Smith
Genetic engineering has the potential to improve plant material in several ways but has multiple drawbacks making it currently impractical for use in the Echinacea industry. Genetic transformation may not improve propagation efficiency, does not necessarily increase yield (except hairy roots), and does not guarantee more standardized plant material. Most importantly, the NHP market may not readily accept the use of GMOs. Despite the fact that ploidy variation and hairy root disease occur naturally, market research should precede employment of such technologies. Genetic transformation is useful to study the growth and biochemistry of plants but, since elicitors and selective breeding can produce similar improvements in yield and quality, genetic engineering may not be the best option for industry.
Plant-made vaccines against parasites: bioinspired perspectives to fight against Chagas disease
Published in Expert Review of Vaccines, 2021
Abel Ramos-Vega, Elizabeth Monreal-Escalante, Eric Dumonteil, Bernardo Bañuelos-Hernández, Carlos Angulo
On the one hand, the choice of genetic transformation can be chosen according to the plant, vector, and delivery method, type of expression, and also on sub-cellular location where the desired expression is planned [71]. Several physical transformation methods are available, of which particle gun (biobalistics) is the most popular. The main advantage of physical methods is that they allow DNA recombination before integration into the plant gene; multiple copies of a foreign gene may be introduced in cells, which results in high expression levels; these methods are still limited for some plants though [72].