Oxidase test – Knowledge and References

Explore chapters and articles related to this topic

Estimation of Bacterial Numbers by Indirect Methods

Published in Maria Csuros, Csaba Csuros, Klara Ver, Microbiological Examination of Water and Wastewater, 2018

The oxidase test indicates the presence of the enzyme cytochrome oxidase. This iron-containing porphyrin enzyme participates in the electron transport mechanism and in the nitrate metabolic pathways of some bacteria. The simple reaction uses the Kovacs method.

Detection of multidrug resistant environmental isolates of acinetobacter and Stenotrophomonas maltophilia: a possible threat for community acquired infections?

View Article

Journal Information

Published in Journal of Environmental Science and Health, Part A, 2020

Reshme Govender, Isaac D Amoah, Sheena Kumari, Faizal Bux, Thor A Stenström

Isolates with typical appearance on their respective medium (red for Acinetobacter, cream colonies for S. maltophilia) were streaked onto fresh selective media for purification. For further purification isolates were subcultured on nutrient agar (Oxoid, England) plates. The cultures were Gram stained and Gram-negative ones were oxidase tested using Oxidase test strips (Sigma Aldrich, South Africa). For the catalase test, a drop of 3% hydrogen peroxide was added to the colonies on a glass-slide and a positive test displayed the formation of bubbles. The presumptive isolates were confirmed by Polymerase chain reaction (PCR) and the identification was further validated by MALDI-TOF (Matrix Assisted Laser Desorption – Time of Flight).